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A4XN72 (SYGB_PSEMY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine--tRNA ligase beta subunit

EC=6.1.1.14
Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS
Gene names
Name:glyS
Ordered Locus Names:Pmen_0013
OrganismPseudomonas mendocina (strain ymp) [Complete proteome] [HAMAP]
Taxonomic identifier399739 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length684 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00255.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glycyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: InterPro

glycine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 684684Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255
PRO_1000006391

Sequences

Sequence LengthMass (Da)Tools
A4XN72 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 639F5C38F18D11A0

FASTA68474,341
        10         20         30         40         50         60 
MSAKDFLVEL GTEELPPKAL KSLGDAFLAG IEKGLKAAGL NYAASRVYAA PRRLAVLVEQ 

        70         80         90        100        110        120 
LEEQQADRSM NLDGPPIQAA FDADGNPTQA ALGFARKCGV DIAEIDRSGA KLRFAQHIPG 

       130        140        150        160        170        180 
QPAVNLLPTI VQDSLNDLPI PKRMRWAARR DEFVRPTQWL VMLFGDAVVD CEILAQKAGR 

       190        200        210        220        230        240 
VSRGHRFHAN REVRISSPAN YAEDLRSAYV LADFAERREI ISRRVDELAA AEQGTAIVPP 

       250        260        270        280        290        300 
ALLDEVTALV EWPVPLVCSF EERFLEVPQE ALISTMQDNQ KYFCLLDAGG KLLPRFITVA 

       310        320        330        340        350        360 
NIESKDPAQI VSGNEKVVRP RLTDAEFFFK QDKKQKLEGF NQRLANVVFQ AQLGSVFDKA 

       370        380        390        400        410        420 
QRVSALAGFI AREVGGDEAR AARAGLLSKC DLATEMVGEF PEMQGIAGYY YALNDGEPQD 

       430        440        450        460        470        480 
VALALNEQYM PRGAGAELPS TLTGAAVAVA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL 

       490        500        510        520        530        540 
GVLRILIEKG LDLDLAAAVD FAVAQYAGKV KSDGLAAQVL EFIFDRLRAR YEDEGIEVAV 

       550        560        570        580        590        600 
YQAVRAVNPT SPLDFDQRVQ AVQAFRKLPQ AEALAAANKR VSNLLSKAEG GVAAQVEAHY 

       610        620        630        640        650        660 
FDNPSEFALH AAIQQADQAV QPLAAARQYN EALAKLASLR EPVDAFFEAV LVNAEDARVR 

       670        680 
ANRYALLARL RGLFLGVADI SVLG 

« Hide

References

[1]"Complete sequence of Pseudomonas mendocina ymp."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Hersman L., Dubois J., Maurice P., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ymp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000680 Genomic DNA. Translation: ABP82788.1.
RefSeqYP_001185520.1. NC_009439.1.

3D structure databases

ProteinModelPortalA4XN72.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399739.Pmen_0013.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP82788; ABP82788; Pmen_0013.
GeneID5106688.
KEGGpmy:Pmen_0013.
PATRIC19906427. VBIPseMen131592_0014.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0751.
HOGENOMHOG000264302.
KOK01879.
OMAMVMAEDE.
OrthoDBEOG661H9S.
ProtClustDBPRK01233.

Enzyme and pathway databases

BioCycPMEN399739:GHR6-13-MONOMER.

Family and domain databases

HAMAPMF_00255. Gly_tRNA_synth_beta.
InterProIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSPR01045. TRNASYNTHGB.
SMARTSM00836. DALR_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR00211. glyS. 1 hit.
PROSITEPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYGB_PSEMY
AccessionPrimary (citable) accession number: A4XN72
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries