Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSAC351627:G1G8P-2209-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:Csac_2154
OrganismiCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331)
Taxonomic identifieri351627 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
Proteomesi
  • UP000000256 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000064631 – 245tRNA (guanine-N(1)-)-methyltransferaseAdd BLAST245

Proteomic databases

PRIDEiA4XLF1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351627.Csac_2154

Structurei

3D structure databases

ProteinModelPortaliA4XLF1
SMRiA4XLF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 136S-adenosyl-L-methionine bindingUniRule annotation6

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D6X Bacteria
COG0336 LUCA
HOGENOMiHOG000016242
KOiK00554
OMAiILCGHYK
OrthoDBiPOG091H01WE

Family and domain databases

Gene3Di1.10.1270.20, 1 hit
3.40.1280.10, 1 hit
HAMAPiMF_00605 TrmD, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR002649 tRNA_m1G_MeTrfase_bac
IPR023148 tRNA_m1G_MeTrfase_C
IPR029026 tRNA_m1G_MTases_N
IPR016009 tRNA_MeTrfase_TRMD/TRM10
PANTHERiPTHR32125:SF1 PTHR32125:SF1, 1 hit
PfamiView protein in Pfam
PF01746 tRNA_m1G_MT, 1 hit
PIRSFiPIRSF000386 tRNA_mtase, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00088 trmD, 1 hit

Sequencei

Sequence statusi: Complete.

A4XLF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFKVLTLFP EVILQATNFS ILKRAQEKGL IKIEAINIRD YTKDKHKRTD
60 70 80 90 100
DYPYGGGFGM VMTAQPIVDA YESIKSSKPH RVIYLTPQGK KYTQDIAKEF
110 120 130 140 150
SKEEELVIIC GHYEGIDQRV IDLIVTDEIS IGDYVLSGGE YAALVLIDSI
160 170 180 190 200
SRLVEGVIEK KSVEEESFSE CLLEYPHYTR PYEFRGLKVP EVLLSGNHEK
210 220 230 240
IKKWRRYQSL LKTIKSRPDL INAANLTKED IEFLIKYCES QKIVL
Length:245
Mass (Da):28,159
Last modified:May 29, 2007 - v1
Checksum:i6D74320E13FA5AD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA Translation: ABP67736.1
RefSeqiWP_011917667.1, NC_009437.1

Genome annotation databases

EnsemblBacteriaiABP67736; ABP67736; Csac_2154
KEGGicsc:Csac_2154

Similar proteinsi

Entry informationi

Entry nameiTRMD_CALS8
AccessioniPrimary (citable) accession number: A4XLF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health