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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei560Aminoacyl-adenylateUniRule annotation1
Binding sitei604ATPUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi893ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1
Metal bindingi913ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCSAC351627:G1G8P-1967-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Csac_1912
OrganismiCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331)
Taxonomic identifieri351627 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
Proteomesi
  • UP000000256 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220521 – 920Isoleucine--tRNA ligaseAdd BLAST920

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi351627.Csac_1912

Structurei

3D structure databases

ProteinModelPortaliA4XKR2
SMRiA4XKR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi601 – 605"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A4XKR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWSQTLNLP KTDFPMRANL AQREPQFLRF WEENDIFKKM LKKNRNNKKF
60 70 80 90 100
ILHDGPPYAN GDIHLGHALN KVLKDIVNKY KSLQGYYTPY IPGWDTHGLP
110 120 130 140 150
IEQQVIKKLG VNRHEVDPVE FRKKCKEFAL SYIDIQRQQF KRLGVFGEWE
160 170 180 190 200
DPYMTLDPKF EARQIRVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE
210 220 230 240 250
YQEDKTYSIY VKFEVIDDKG LFANLNLSGK KVYIVIWTTT TWTLPGNLAI
260 270 280 290 300
ALNTDFDYSL VDVGNEILIV ASELVERVMK TNKIDQYHEI ARFKGKDLEY
310 320 330 340 350
VKCKHPFLDR TSLVILGEHV TLEAGTGCVH TAPGHGEEDF EVCERYNIPV
360 370 380 390 400
IVPVDNKGYL TQEAGKFAGL FYEDSNKEIA KELEASDHLL GVEKITHQYP
410 420 430 440 450
HCWRCKNPVI FRATEQWFAS VKGFREEALK AVDDVKWVPE WGRDRIYNMI
460 470 480 490 500
ADRQDWCISR QRIWGVPIPI FYCKNCRKEL ITDETIDHIA KIFEKEGSDA
510 520 530 540 550
WFSKDVKELL PEGTKCPVCG CMEFEKETDI MDVWFDSGSS HAYVLESRED
560 570 580 590 600
LEWPCDMYLE GNDQYRGWFQ SSLLTAVATK GRAPYRIVLT HGFVVDGEGK
610 620 630 640 650
KMSKSEGNVI SPFDIINEFG ADILRLWCVS ADYTTDMRIS KDIIKQLTEI
660 670 680 690 700
YRKIRNTARF LLGNLYDFNP KTDKVGCENL KEIDKWALQR LYKLIEKVTK
710 720 730 740 750
AYEEYDYNQV YHLVHNFCVI DMSNLYLDIN KDRLYASKSE SLDRRSAQTV
760 770 780 790 800
MYEILVALTK LIAPILSFTA EEIWQNIIFK EEDAESVFLT SWPKVNENIL
810 820 830 840 850
KDETLEEKWN KIIEIKDIVA KQLEIARNEK LIGSSLDSKV KIFAKGNIKR
860 870 880 890 900
FIEENKDIIQ EVLIVSQLEV EEGDSDQIKV EVYKADGLKC ERCWKFDTMV
910 920
GKNEENLNVC PRCYEVVKVK
Length:920
Mass (Da):107,030
Last modified:May 29, 2007 - v1
Checksum:iC35E80AF111A9BFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA Translation: ABP67497.1
RefSeqiWP_011917433.1, NC_009437.1

Genome annotation databases

EnsemblBacteriaiABP67497; ABP67497; Csac_1912
KEGGicsc:Csac_1912

Similar proteinsi

Entry informationi

Entry nameiSYI_CALS8
AccessioniPrimary (citable) accession number: A4XKR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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