Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A4XKR2

- SYI_CALS8

UniProt

A4XKR2 - SYI_CALS8

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
Binding sitei604 – 6041ATPUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCSAC351627:GJ17-1953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Csac_1912
OrganismiCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903)
Taxonomic identifieri351627 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
ProteomesiUP000000256: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Isoleucine--tRNA ligasePRO_1000022052Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi351627.Csac_1912.

Structurei

3D structure databases

ProteinModelPortaliA4XKR2.
SMRiA4XKR2. Positions 1-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi601 – 6055"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4XKR2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDWSQTLNLP KTDFPMRANL AQREPQFLRF WEENDIFKKM LKKNRNNKKF
60 70 80 90 100
ILHDGPPYAN GDIHLGHALN KVLKDIVNKY KSLQGYYTPY IPGWDTHGLP
110 120 130 140 150
IEQQVIKKLG VNRHEVDPVE FRKKCKEFAL SYIDIQRQQF KRLGVFGEWE
160 170 180 190 200
DPYMTLDPKF EARQIRVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE
210 220 230 240 250
YQEDKTYSIY VKFEVIDDKG LFANLNLSGK KVYIVIWTTT TWTLPGNLAI
260 270 280 290 300
ALNTDFDYSL VDVGNEILIV ASELVERVMK TNKIDQYHEI ARFKGKDLEY
310 320 330 340 350
VKCKHPFLDR TSLVILGEHV TLEAGTGCVH TAPGHGEEDF EVCERYNIPV
360 370 380 390 400
IVPVDNKGYL TQEAGKFAGL FYEDSNKEIA KELEASDHLL GVEKITHQYP
410 420 430 440 450
HCWRCKNPVI FRATEQWFAS VKGFREEALK AVDDVKWVPE WGRDRIYNMI
460 470 480 490 500
ADRQDWCISR QRIWGVPIPI FYCKNCRKEL ITDETIDHIA KIFEKEGSDA
510 520 530 540 550
WFSKDVKELL PEGTKCPVCG CMEFEKETDI MDVWFDSGSS HAYVLESRED
560 570 580 590 600
LEWPCDMYLE GNDQYRGWFQ SSLLTAVATK GRAPYRIVLT HGFVVDGEGK
610 620 630 640 650
KMSKSEGNVI SPFDIINEFG ADILRLWCVS ADYTTDMRIS KDIIKQLTEI
660 670 680 690 700
YRKIRNTARF LLGNLYDFNP KTDKVGCENL KEIDKWALQR LYKLIEKVTK
710 720 730 740 750
AYEEYDYNQV YHLVHNFCVI DMSNLYLDIN KDRLYASKSE SLDRRSAQTV
760 770 780 790 800
MYEILVALTK LIAPILSFTA EEIWQNIIFK EEDAESVFLT SWPKVNENIL
810 820 830 840 850
KDETLEEKWN KIIEIKDIVA KQLEIARNEK LIGSSLDSKV KIFAKGNIKR
860 870 880 890 900
FIEENKDIIQ EVLIVSQLEV EEGDSDQIKV EVYKADGLKC ERCWKFDTMV
910 920
GKNEENLNVC PRCYEVVKVK
Length:920
Mass (Da):107,030
Last modified:May 29, 2007 - v1
Checksum:iC35E80AF111A9BFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA. Translation: ABP67497.1.
RefSeqiYP_001180688.1. NC_009437.1.

Genome annotation databases

EnsemblBacteriaiABP67497; ABP67497; Csac_1912.
GeneIDi5088631.
KEGGicsc:Csac_1912.
PATRICi21253572. VBICalSac56748_2092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA. Translation: ABP67497.1 .
RefSeqi YP_001180688.1. NC_009437.1.

3D structure databases

ProteinModelPortali A4XKR2.
SMRi A4XKR2. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 351627.Csac_1912.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP67497 ; ABP67497 ; Csac_1912 .
GeneIDi 5088631.
KEGGi csc:Csac_1912.
PATRICi 21253572. VBICalSac56748_2092.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CSAC351627:GJ17-1953-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43494 / DSM 8903.

Entry informationi

Entry nameiSYI_CALS8
AccessioniPrimary (citable) accession number: A4XKR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: November 26, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3