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A4XKG9 (SYL_CALS8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Csac_1819
OrganismCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]
Taxonomic identifier351627 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

Protein attributes

Sequence length817 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 817817Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009315

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif578 – 5825"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5811ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4XKG9 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 01C44C1BC39859F1

FASTA81795,200
        10         20         30         40         50         60 
MEYNFREIEK KWQQKWFSQN KYKVTEDSPK PKYYVLEMFP YPSGKLHMGH VRNYSIGDVF 

        70         80         90        100        110        120 
ARFMRLKGYN VLHPMGWDAF GLPAENAAIK HGIHPSDWTW SNIENMKRQL KELGISYDWD 

       130        140        150        160        170        180 
REVATCHPDY YKWTQWMFLQ FYKAGLAYRK RSYVNWCPSC ETVLANEQVV NGRCERCKSL 

       190        200        210        220        230        240 
VGKKDLEQWF FRITKYAERL LRDIDKLDGW PEKVKIMQKN WIGRSEGAEI EFEIDGLGKR 

       250        260        270        280        290        300 
IKVFTTRPDT LFGVTYLVLA PEHPLTKEII AGKPQEKECL EFIEKMQYLN EIERTSTETE 

       310        320        330        340        350        360 
KEGRFTGGYA IHPLTGKKVP IYIANYVLVD YGTGAVMGVP AHDQRDFEFA KKYNLPIKVV 

       370        380        390        400        410        420 
IKGDGVDIQN LQSAYEGEGV LINSGEFNGL KNTEAMKKIT EYLEKNGYGK ACVTYKLRDW 

       430        440        450        460        470        480 
LISRQRYWGA PIPIVYCDDC GIVPVPEEEL PVLLPYNVEF KPTGQSPLAY CEEFVNTTCP 

       490        500        510        520        530        540 
KCGKPARRET DTMDTFICSS WYYFRYTDPK NSEKPFEKSL VDYWLPVDQY IGGVEHAILH 

       550        560        570        580        590        600 
LLYSRFFTKV LYDLGYISFE EPFKNLLTQG MVLKDGAKMS KSLGNIVSPE EIVEKYGADT 

       610        620        630        640        650        660 
ARLFILFAAP PERDLEWSDQ GVEGCFRFLN RLWRLYIELK DKLSDSSLPG QSELDDELNY 

       670        680        690        700        710        720 
RLNYTIKKVT EDIGERFNFN TAISSIMELL NFLYDYKEKG SLNRELILKT LKNFLILIYP 

       730        740        750        760        770        780 
FTPHIACELW EIMGFEGDIE DVSWPEYDES ALVRKNVEIA VQINGKVRAR FDVPVDISEE 

       790        800        810 
ELKQKIMNNE KIKTLLEGKE IVKFIYVKNR LVNIVIK 

« Hide

References

[1]"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43494 / DSM 8903.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000679 Genomic DNA. Translation: ABP67404.1.
RefSeqYP_001180595.1. NC_009437.1.

3D structure databases

ProteinModelPortalA4XKG9.
SMRA4XKG9. Positions 2-752.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351627.Csac_1819.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP67404; ABP67404; Csac_1819.
GeneID5086638.
KEGGcsc:Csac_1819.
PATRIC21253376. VBICalSac56748_1994.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCSAC351627:GJ17-1858-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CALS8
AccessionPrimary (citable) accession number: A4XKG9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries