Reviewed, UniProtKB/Swiss-Prot A4XK60 (PROA_CALS8)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Csac_1708

Organism

Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]

Taxonomic identifier

351627 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049941

Sequences

	Sequence		Length	Mass (Da)
	A4XK60-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: BA28441D77DE9D1D	FASTA	419	46,002
	10 20 30 40 50 60 MSDLIQKAQK VKEASKKLMN LSESQKNLAL SCISKKILDN MEYILVENKK DMENAQNKGI 70 80 90 100 110 120 KGALLDRLKL TEDRIRQICK GIEDVIKLPD PVGEVISMWK RPNGLIIGQK RVPIGAIGII 130 140 150 160 170 180 YEARPNVTVD AAVLCLKAGN SVLLRGGSEA INSNVALVKT MKEGLIEAGI DEGSIEIVED 190 200 210 220 230 240 TSRETAVAMM KLNEYLDLLI PRGGANLIKT VVQNATVPVI ETGVGNCHVF VDESADFEMA 250 260 270 280 290 300 EKIVINAKTQ RPGVCNAAEK LLVHKNIAES FLPMIVKKLM TKGVEIRGCS KTVEICKQNG 310 320 330 340 350 360 IEVKEATEDD WYTEYLDLII GVKVVDSIDA AIEHINKYGS KHSEAIVTRD YFNAQKFLDF 370 380 390 400 410 VDAAACYVNA STRFTDGFEF GFGAEIGIST QKLHARGPMG LKELTTIKYI ILGSGQVRE

References

[1]

"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000679 Genomic DNA. Translation: ABP67295.1.
RefSeq	YP_001180486.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5088167.
GenomeReviews	Gene locus Csac_1708 in contig CP000679_GR.
KEGG	csc:Csac_1708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4XK60. QYPAACN.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016162. Ald_DH_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CALS8

Accession

Primary (citable) accession number: A4XK60

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)