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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101ATPUniRule annotation
Binding sitei148 – 1481ATPUniRule annotation
Binding sitei187 – 1871ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi179 – 1824ATPUniRule annotation
Nucleotide bindingi266 – 2672ATPUniRule annotation

GO - Molecular functioni

  1. 5-(carboxyamino)imidazole ribonucleotide synthase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: InterPro
  4. phosphoribosylaminoimidazole carboxylase activity Source: InterPro

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSAC351627:GJ17-1667-MONOMER.
UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotation
Ordered Locus Names:Csac_1629Imported
OrganismiCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903)Imported
Taxonomic identifieri351627 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
ProteomesiUP000000256 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351627.Csac_1629.

Structurei

3D structure databases

ProteinModelPortaliA4XJY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 296183ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0026.
HOGENOMiHOG000034028.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiEOG6QZMX7.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4XJY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKGVFGYPL MKIGIIGGGQ LGKMLSQKAK QMGFYVISLD PSAACPAASV
60 70 80 90 100
SDELIVSDFY SPEKLKELVE KSDITTYEIE HINTSVLKEL YDEGYNILPS
110 120 130 140 150
PYCLEIIQDK LKQKQVLQSA GLPVPRFERV ESFDISFFEN FGFPLVQKAT
160 170 180 190 200
KGGYDGRGVV VLKSKDDINK VLKTESYIEE FVDVEKELAV IVARNKKGDV
210 220 230 240 250
VSYPVVEMVF DETANILDIL IVPARVEKDI EDEAKKIAIK AVEALQGVGV
260 270 280 290 300
FGVELFLTKD RKILINEIAP RPHNSGHYTI EACITSQFEQ HLRAICDLPL
310 320 330 340 350
GSTKLLSPAV MINLLGDDGY KGTPVIEGLI DALSIEGVSF HFYGKKVTAP
360 370 380
FRKMGHVTIL DDNLERAIEK AKKVKEVLKI KSEV
Length:384
Mass (Da):42,589
Last modified:May 29, 2007 - v1
Checksum:iCC1F140EE4EECC9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA. Translation: ABP67219.1.
RefSeqiYP_001180410.1. NC_009437.1.

Genome annotation databases

EnsemblBacteriaiABP67219; ABP67219; Csac_1629.
KEGGicsc:Csac_1629.
PATRICi21252974. VBICalSac56748_1796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA. Translation: ABP67219.1.
RefSeqiYP_001180410.1. NC_009437.1.

3D structure databases

ProteinModelPortaliA4XJY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351627.Csac_1629.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP67219; ABP67219; Csac_1629.
KEGGicsc:Csac_1629.
PATRICi21252974. VBICalSac56748_1796.

Phylogenomic databases

eggNOGiCOG0026.
HOGENOMiHOG000034028.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiEOG6QZMX7.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.
BioCyciCSAC351627:GJ17-1667-MONOMER.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43494 / DSM 8903Imported.

Entry informationi

Entry nameiA4XJY4_CALS8
AccessioniPrimary (citable) accession number: A4XJY4
Entry historyi
Integrated into UniProtKB/TrEMBL: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 29, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.