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A4XIU2 (FTHS_CALS8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Csac_1224
OrganismCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]
Taxonomic identifier351627 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000318565

Regions

Nucleotide binding64 – 718ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4XIU2 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: CADABD9B733011CB

FASTA55460,246
        10         20         30         40         50         60 
MKSTSKLQEM KLKNITEIAE SVGLSEDDIE LYGKYKAKIS LDVLKQKPRQ REGKVILVTS 

        70         80         90        100        110        120 
INPTPYGEGK TTTAIGLSMA INRLGFKSIV TLREPSLGPY LGIKGGATGG GVAQVLPSTD 

       130        140        150        160        170        180 
INLHFTGDIH AVTSANNLLC AAIDNHIYHG NELNINPKAV MVKRAMDMND RALRNIVIGL 

       190        200        210        220        230        240 
GDGQRGAVRE DGFIISVASE VMAILCLSND LEDLKERLGN ILVGFSYDKK PIYAKDLKVH 

       250        260        270        280        290        300 
GAMALLLKDA IKPNLVQTSE ATAAIIHGGP FANIAHGTNS IIAIKIAQKL SDYVVVEAGF 

       310        320        330        340        350        360 
GADLGAEKFV NIVSRKSGIY PSAAVMVVTT KALKYHGSMG AKENLTSENI DTLKKGFKNL 

       370        380        390        400        410        420 
EKHIENLKLL GLEAIVTLNR FPHDTPAEIS EIESFCKERG VEFAVSEAYE LGSEGALDLA 

       430        440        450        460        470        480 
QKVIEVASRK RKINFVYEDS DPVEEKIRKV AKTIYGAADV QFSKSALLGL ELIKKLNIDH 

       490        500        510        520        530        540 
FPICMAKTQY SLSDDPKLLG RPKDFVLNVN EIRINNGAQF IVVICGDIMT MPGLSKDYAA 

       550 
LHLDIDEDGN VVWV 

« Hide

References

[1]"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43494 / DSM 8903.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000679 Genomic DNA. Translation: ABP66827.1.
RefSeqYP_001180018.1. NC_009437.1.

3D structure databases

ProteinModelPortalA4XIU2.
SMRA4XIU2. Positions 7-552.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351627.Csac_1224.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP66827; ABP66827; Csac_1224.
GeneID5087440.
KEGGcsc:Csac_1224.
PATRIC21252104. VBICalSac56748_1366.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMAIANSVSM.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycCSAC351627:GJ17-1253-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFTHS_CALS8
AccessionPrimary (citable) accession number: A4XIU2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways