Reviewed, UniProtKB/Swiss-Prot A4XIU2 (FTHS_CALS8)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Csac_1224

Organism

Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]

Taxonomic identifier

351627 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000318565
Regions
	Nucleotide binding	64 – 71	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4XIU2-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: CADABD9B733011CB	FASTA	554	60,246
	10 20 30 40 50 60 MKSTSKLQEM KLKNITEIAE SVGLSEDDIE LYGKYKAKIS LDVLKQKPRQ REGKVILVTS 70 80 90 100 110 120 INPTPYGEGK TTTAIGLSMA INRLGFKSIV TLREPSLGPY LGIKGGATGG GVAQVLPSTD 130 140 150 160 170 180 INLHFTGDIH AVTSANNLLC AAIDNHIYHG NELNINPKAV MVKRAMDMND RALRNIVIGL 190 200 210 220 230 240 GDGQRGAVRE DGFIISVASE VMAILCLSND LEDLKERLGN ILVGFSYDKK PIYAKDLKVH 250 260 270 280 290 300 GAMALLLKDA IKPNLVQTSE ATAAIIHGGP FANIAHGTNS IIAIKIAQKL SDYVVVEAGF 310 320 330 340 350 360 GADLGAEKFV NIVSRKSGIY PSAAVMVVTT KALKYHGSMG AKENLTSENI DTLKKGFKNL 370 380 390 400 410 420 EKHIENLKLL GLEAIVTLNR FPHDTPAEIS EIESFCKERG VEFAVSEAYE LGSEGALDLA 430 440 450 460 470 480 QKVIEVASRK RKINFVYEDS DPVEEKIRKV AKTIYGAADV QFSKSALLGL ELIKKLNIDH 490 500 510 520 530 540 FPICMAKTQY SLSDDPKLLG RPKDFVLNVN EIRINNGAQF IVVICGDIMT MPGLSKDYAA 550 LHLDIDEDGN VVWV

References

[1]

"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000679 Genomic DNA. Translation: ABP66827.1.
RefSeq	YP_001180018.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4XIU2.
Genome annotation databases
GeneID	5087440.
GenomeReviews	Gene locus Csac_1224 in contig CP000679_GR.
KEGG	csc:Csac_1224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KMPGLPK.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. False negative. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CALS8

Accession

Primary (citable) accession number: A4XIU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)