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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSAC351627:GJ17-1253-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:Csac_1224
OrganismiCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903)
Taxonomic identifieri351627 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
ProteomesiUP000000256 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Formate--tetrahydrofolate ligasePRO_0000318565Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351627.Csac_1224.

Structurei

3D structure databases

SMRiA4XIU2. Positions 7-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiMDMNDRQ.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

A4XIU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSTSKLQEM KLKNITEIAE SVGLSEDDIE LYGKYKAKIS LDVLKQKPRQ
60 70 80 90 100
REGKVILVTS INPTPYGEGK TTTAIGLSMA INRLGFKSIV TLREPSLGPY
110 120 130 140 150
LGIKGGATGG GVAQVLPSTD INLHFTGDIH AVTSANNLLC AAIDNHIYHG
160 170 180 190 200
NELNINPKAV MVKRAMDMND RALRNIVIGL GDGQRGAVRE DGFIISVASE
210 220 230 240 250
VMAILCLSND LEDLKERLGN ILVGFSYDKK PIYAKDLKVH GAMALLLKDA
260 270 280 290 300
IKPNLVQTSE ATAAIIHGGP FANIAHGTNS IIAIKIAQKL SDYVVVEAGF
310 320 330 340 350
GADLGAEKFV NIVSRKSGIY PSAAVMVVTT KALKYHGSMG AKENLTSENI
360 370 380 390 400
DTLKKGFKNL EKHIENLKLL GLEAIVTLNR FPHDTPAEIS EIESFCKERG
410 420 430 440 450
VEFAVSEAYE LGSEGALDLA QKVIEVASRK RKINFVYEDS DPVEEKIRKV
460 470 480 490 500
AKTIYGAADV QFSKSALLGL ELIKKLNIDH FPICMAKTQY SLSDDPKLLG
510 520 530 540 550
RPKDFVLNVN EIRINNGAQF IVVICGDIMT MPGLSKDYAA LHLDIDEDGN

VVWV
Length:554
Mass (Da):60,246
Last modified:May 29, 2007 - v1
Checksum:iCADABD9B733011CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA. Translation: ABP66827.1.
RefSeqiYP_001180018.1. NC_009437.1.

Genome annotation databases

EnsemblBacteriaiABP66827; ABP66827; Csac_1224.
KEGGicsc:Csac_1224.
PATRICi21252104. VBICalSac56748_1366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA. Translation: ABP66827.1.
RefSeqiYP_001180018.1. NC_009437.1.

3D structure databases

SMRiA4XIU2. Positions 7-552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351627.Csac_1224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP66827; ABP66827; Csac_1224.
KEGGicsc:Csac_1224.
PATRICi21252104. VBICalSac56748_1366.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiMDMNDRQ.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCSAC351627:GJ17-1253-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43494 / DSM 8903.

Entry informationi

Entry nameiFTHS_CALS8
AccessioniPrimary (citable) accession number: A4XIU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: April 1, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.