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A4XIM9 (MURB_CALS8) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158
Alternative name(s):
UDP-N-acetylmuramate dehydrogenase
Gene names
Name:murB
Ordered Locus Names:Csac_1159
OrganismCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]
Taxonomic identifier351627 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP MF_00037

Catalytic activity

UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037

Cofactor

FAD By similarity. HAMAP MF_00037

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037

Subcellular location

Cytoplasm By similarity HAMAP MF_00037.

Sequence similarities

Belongs to the MurB family.

Contains 1 FAD-binding PCMH-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037
PRO_0000332452

Regions

Domain27 – 192166FAD-binding PCMH-type

Sites

Active site1711 By similarity
Active site2231Proton donor By similarity
Active site2931 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4XIM9 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: D422E97F0777D187

FASTA31034,981
        10         20         30         40         50         60 
MKFERGLEKL NIEFLKDKPL KDFTTFKIGG KARYIVFPKN IDELIEIIKL VKESGINWRI 

        70         80         90        100        110        120 
VGNCSNVLVS DKGFDGAIIT TTKMDFFKTE ENLIEAECGC MISQVARKAC ENGLKGLEFA 

       130        140        150        160        170        180 
VGIPGTVGGA VYMNAGAYDS EIKDVFECAE VLDEDLNIFK LGKSDMRFSY RHSRLKEEKL 

       190        200        210        220        230        240 
ILLKATFRLQ YAREEDVPPI EKANEYNQRR REKQPLQYPS AGSIFKRPPG NFAGKLIEDA 

       250        260        270        280        290        300 
GLKGYRVGNA CISGKHAGFI VNLGDALAED VRKLIYHTQK SVYEKFGVLL EPEIEFIGEF 

       310 
ETPLFEMSTK

« Hide

References

[1]"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43494 / DSM 8903.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000679 Genomic DNA. Translation: ABP66764.1.
RefSeqYP_001179955.1. NC_009437.1.

3D structure databases

ProteinModelPortalA4XIM9.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4XIM9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5086663.
GenomeReviewsGene locus Csac_1159 in contig CP000679_GR.
KEGGcsc:Csac_1159.
PATRIC21251974. VBICalSac56748_1302.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0812.
HOGENOMHBG686573.
OMAISERHAN.
ProtClustDBPRK13905.

Family and domain databases

HAMAPMF_00037. MurB.
[Tree]
InterProIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
Gene3DG3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit.
G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit.
G3DSA:3.90.78.10. MurB_C. 1 hit.
KOK00075.
PANTHERPTHR21071. MurB. 1 hit.
PfamPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMSSF56176. FAD-binding_2. 1 hit.
SSF56194. MurB_C. 1 hit.
TIGRFAMsTIGR00179. MurB. 1 hit.
PROSITEPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMURB_CALS8
AccessionPrimary (citable) accession number: A4XIM9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 29, 2007
Last modified: December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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