A4XHL8 (A4XHL8_CALS8) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	Csac_0784

Organism

Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]

Taxonomic identifier

351627 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	318	1	Nucleophile By similarity HAMAP MF_00685
	Active site	365	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	A4XHL8 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 7797E370E4844560	FASTA	645	76,254
	10 20 30 40 50 60 MIKKVKSTIY LSDIKRFEAG EHFESYKFLG SRVVNYRGKV GTVFCVWAPN AKSVSVVGDF 70 80 90 100 110 120 NNWCGKNHKM MRVHGSGFWW LFVEGLLEGE LYKYEIIGAD GKRVLKADPY AIYSEVRPNT 130 140 150 160 170 180 ASIVKNIPDY EWHDHEWMEK RKVTPPYDKP INIYEVHLAS WKMKKDGTVE KAGEFYNYRE 190 200 210 220 230 240 LAHMLVDYLK VMNYTHIELL PVLEHPLDMS WGYQPTGYFS VTSRYGCPED FMYFVDIMHQ 250 260 270 280 290 300 NGIGVIVDWV PAHFCKDEHG LYRFDGTFLY EYEDELLREN YTWGTATFDF SKPQVQSFLI 310 320 330 340 350 360 SSAMFWFDIY HIDGIRVDAV SHIIYMNNNQ KNRYGGHENI EGIEFIKKLN KAIFSKYPNI 370 380 390 400 410 420 LMIAEESTAF PLVTYPTYDG GLGFNYKWNM GWMNDTLKYM QFYPDERKYH HNLLTFSIMY 430 440 450 460 470 480 AFSENFILPF SHDEVVHGKK SLLDKMPGEY NQKFANLRLL YGYMYTHPGK KLLFMGSEFG 490 500 510 520 530 540 QFIEWRFYAS LDWLLLDYPM HRMLQHYVKS LNRFYLENKA LWELDHKMDG FRWIDVHNWE 550 560 570 580 590 600 QSVISYLRFS KDPDDFLVVI CNFGLASYEN YKIGVPRKGI YMEVFNSDKA EFGGNNIVNT 610 620 630 640 EKLKTIDEVW HGYPQCIEFR LPSLSCLIFK PVELFEDTKE KATDE

References

[1]

"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43494 / DSM 8903.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000679 Genomic DNA. Translation: ABP66403.1.
RefSeq	YP_001179594.1. NC_009437.1.
3D structure databases
ProteinModelPortal	A4XHL8.
ModBase	Search...
Protein-protein interaction databases
STRING	A4XHL8.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5087657.
GenomeReviews	Gene locus Csac_0784 in contig CP000679_GR.
KEGG	csc:Csac_0784.
PATRIC	21251140. VBICalSac56748_0892.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	WRARINT.
ProtClustDB	CLSK2472773.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4XHL8_CALS8

Accession

Primary (citable) accession number: A4XHL8

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)