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A4XGB8 (BIOB_CALS8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Csac_0307
OrganismCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]
Taxonomic identifier351627 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Biotin synthase HAMAP-Rule MF_01694
PRO_0000381279

Sites

Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1171Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1491Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2091Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A4XGB8 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: A9E7096310F5FA68

FASTA33037,494
        10         20         30         40         50         60 
MLNFLQSVQF AKEIEKKIIE YEQDIDISEA TMLYEIAKHD FDLVKNLADT IKRYYFKNNI 

        70         80         90        100        110        120 
ELCSIYPAKV GMCTEDCKFC SQSIYHSCKI EIKDVASVDE VIEYLENIIA FPVKRFSLVT 

       130        140        150        160        170        180 
SGGELNDSEF ERIIDIISYI SKNYDIHLCA SLGFLTKERA KKLMLAGVTR YHNNLETSST 

       190        200        210        220        230        240 
YFKNICSTHT QAQKIETLKI AKETGLEICS GGIISMGEDM IERIKLAFEL RELKVDSVPI 

       250        260        270        280        290        300 
NILNPIKGTP FEDMKIMDQN EIFITLALFR IVLPRKTILL AGGKENALKD MEKIAYKCGI 

       310        320        330 
NGCMVGNYLT TQGMGIKEKV EMLESLGLEV 

« Hide

References

[1]"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43494 / DSM 8903.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000679 Genomic DNA. Translation: ABP65953.1.
RefSeqYP_001179144.1. NC_009437.1.

3D structure databases

ProteinModelPortalA4XGB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351627.Csac_0307.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP65953; ABP65953; Csac_0307.
GeneID5087248.
KEGGcsc:Csac_0307.
PATRIC21250046. VBICalSac56748_0357.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycCSAC351627:GJ17-317-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CALS8
AccessionPrimary (citable) accession number: A4XGB8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways