A4XGB6 (BIOD_CALS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	Csac_0305

Organism

Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) [Complete proteome] [HAMAP]

Taxonomic identifier

351627 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000019555
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
	Nucleotide binding	115 – 118	4	ATP By similarity
	Nucleotide binding	179 – 180	2	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	54	1	Magnesium 2 By similarity
	Metal binding	115	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	54	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4XGB6 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: AA98ED64A33E871F	FASTA	243	27,577
	10 20 30 40 50 60 MKAVYIIGTD TDVGKTLICA GLCWALKEKG YNIGYFKPVL SGAKRRGKML IPQDTEFVVN 70 80 90 100 110 120 FAKIKGDIYR LTPFIFEKPA SPHIAASDEN VDINVNQIKQ TFEDLSQNYE FVIIEGCGGL 130 140 150 160 170 180 AVPLKEERNQ FYMQYQLIKE ICNNVILVTT TKLGTINHTL LTVEFAKAYG LCLKGIIVNM 190 200 210 220 230 240 YKNEPDEDKV INTITKFTNI PILAKVDFIN DFPSDVDENK FKNVFKKCFD DRAIRKIMGV FEC

References

[1]

"Genome sequence of the thermophilic hydrogen-producing bacterium Caldicellulosiruptor saccharolyticus DSM 8903." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L., Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M., Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43494 / DSM 8903.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000679 Genomic DNA. Translation: ABP65951.1.
RefSeq	YP_001179142.1. NC_009437.1.
3D structure databases
ProteinModelPortal	A4XGB6.
ModBase	Search...
Protein-protein interaction databases
STRING	A4XGB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5087246.
GenomeReviews	Gene locus Csac_0305 in contig CP000679_GR.
KEGG	csc:Csac_0305.
PATRIC	21250042. VBICalSac56748_0355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	LNNYNHE.
ProtClustDB	CLSK852384.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CALS8

Accession

Primary (citable) accession number: A4XGB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program