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A4X9S8 (SYL_SALTO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Strop_3225
OrganismSalinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]
Taxonomic identifier369723 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeSalinispora

Protein attributes

Sequence length954 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 954954Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091362

Regions

Motif67 – 7812"HIGH" region HAMAP-Rule MF_00049
Motif729 – 7335"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4X9S8 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 46A367CA446A4CB6

FASTA954106,010
        10         20         30         40         50         60 
MTEAAAPASD IPPFRYTADL ADEIERRWQD TWEREGTFHA PNPTGPLADP EHPRAGAEKL 

        70         80         90        100        110        120 
YVLDMFPYPS GAGLHVGHPL GYIGTDCFAR YQRMAGRNVL HAMGFDAFGL PAEQYAVQTG 

       130        140        150        160        170        180 
THPRTTTEAN IARYKAQLRR LGLAHDERRS VATIDADFYR WTQWVFLQIY NAWYDSSAKR 

       190        200        210        220        230        240 
ARPIAELVAE FSGGSRRTPD GRPWGELTDA ERRAVVDQHR LAYVSQAPVN WCPGLGTVLA 

       250        260        270        280        290        300 
NEEVTADGRS ERGNFPVFKR NLKQWMMRIT AYGDRLLDDL EKLDWPEPIK LMQRNWIGRS 

       310        320        330        340        350        360 
TGAHIEFPTS APDSDAEGEP RISVFTTRPD TIFGATYLVL APEHDLVDTL VPTAWPAGVP 

       370        380        390        400        410        420 
QAWTGGQASP REAVAGYRKV AAAKTDLERQ AETKEKTGVF IGSYATNPVT GAQIPIFIAD 

       430        440        450        460        470        480 
YVLAGYGTGA IMAVPGQDER DWAFAEVFDL PIVRTVQPAE GFAGKAYTGD GLAINSATPE 

       490        500        510        520        530        540 
RGLDLNGLGV AEAKARTIAW LEAGGHGSGA VTYRLRDWLF SRQRYWGEPF PIVYDETGAA 

       550        560        570        580        590        600 
IALPEELLPV ELPEVDDFAP RTFDPSDAES NPETPLSRRR DWVEVELDLG DGPKRYTRET 

       610        620        630        640        650        660 
NVMPQWAGSC WYELRYLDPT NGDRFVDPEA ERYWMGPRGE GDCGGTDLYV GGAEHAVLHL 

       670        680        690        700        710        720 
LYARFWHKVL YDLGHVSSFE PFRKLFNQGY IQAYAYTDAR GAYVPAEEVV ERSGTYYLGD 

       730        740        750        760        770        780 
VQVNREYGKM GKSLRNVVTP DEMCAAYGAD TFRVYEMSMG PLEVSRPWET RAVVGSFRFL 

       790        800        810        820        830        840 
QRVWRAIVDE RSGASRVVDV PADEATRRLL HRIVDGVRGD MEAMRFNTTI AKLIELTNAL 

       850        860        870        880        890        900 
TRLPETPREV AEPLVLMLAP FAPHVAEELW RRMGHETSLT YADFPVADPA LLVAESVTYP 

       910        920        930        940        950 
VQVNGKVRGR IEVPADAGQE TVRAAALEAV AASLAGKEPR KVIVVPGRMV SVVA 

« Hide

References

[1]"Complete sequence of Salinispora tropica CNB-440."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000667 Genomic DNA. Translation: ABP55659.1.
RefSeqYP_001160037.1. NC_009380.1.

3D structure databases

ProteinModelPortalA4X9S8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING369723.Strop_3225.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP55659; ABP55659; Strop_3225.
GeneID5059690.
KEGGstp:Strop_3225.
PATRIC23445554. VBISalTro43511_3317.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAYARYHRM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSTRO369723:GI49-3261-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SALTO
AccessionPrimary (citable) accession number: A4X9S8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries