Reviewed, UniProtKB/Swiss-Prot A4X776 (FOLD1_SALTO)

Names and origin

Protein names

Recommended name:
    Bifunctional protein folD 1
Including the following 2 domains:
    1- Recommended name:
            Methylenetetrahydrofolate dehydrogenase
              EC=1.5.1.5
    2- Recommended name:
            Methenyltetrahydrofolate cyclohydrolase
              EC=3.5.4.9

Gene names

Name:	folD1
Ordered Locus Names:	Strop_2276

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH. 5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Bifunctional protein folD 1		PRO_0000340595

Sequences

	Sequence		Length	Mass (Da)
	A4X776-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 6E9198E30FB86A3A	FASTA	306	31,813
	10 20 30 40 50 60 MSTSMSVARS LPGGPVAEQI LAEVAQEVTQ LRAAGVTPAL ATVLVGDDDA SVGYIRIKQR 70 80 90 100 110 120 QAVQLGFASP HVRLPASASQ ADLQAVVTQL SADTSVHGVL VQHPVPPHLD FDRAMQALDP 130 140 150 160 170 180 EKDVDGMHPV NLGRLALGLP GPTPCTPAGI EALLAYHEVP VAGREVVVLG RGATLGRPLA 190 200 210 220 230 240 MVLAQKRPTA NAAVTVVHTG VPDWPRYTRR AEIVVAAAGV PGLVRPEHLR PGAVVVNGGV 250 260 270 280 290 300 RYAGRRLLPD VDESCATVAG AMTPRVGGVG PTTVAMLFRN AVRAARRTRS SRTPVRLPDS GAPAGR

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000667 Genomic DNA. Translation: ABP54726.1.
RefSeq	YP_001159104.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5058739.
GenomeReviews	Gene locus Strop_2276 in contig CP000667_GR.
KEGG	stp:Strop_2276.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01576. [Tree]
InterPro	IPR000672. THF_DHase/CycOHase. [Graphical view]
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProDom	PD002300. THFDhg/Cyc_hydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00766. THF_DHG_CYH_1. False negative. PS00767. THF_DHG_CYH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD1_SALTO

Accession

Primary (citable) accession number: A4X776

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)