A4X6K7 (KATG_SALTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Strop_2054

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	Catalase-peroxidase HAMAP MF_01961		PRO_0000354903
Sites
	Active site	129	1	Proton acceptor By similarity
	Metal binding	291	1	Iron (heme axial ligand) By similarity
	Site	125	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	128 ↔ 250		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-276) By similarity
	Cross-link	250 ↔ 276		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-128) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4X6K7 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 8806AAC7990C19F3	FASTA	758	82,880
	10 20 30 40 50 60 MSDTQDNAPV SAQGVDQKAA AGCPVAHDSV TAHGSESESP AIDSPSAVGG GRPRTNRDWW 70 80 90 100 110 120 PNQLDLSVLS TNSAKVNPLG EDFSYAKEFA KLDVEALKRD ITEVLTTSQD WWPADFGHYG 130 140 150 160 170 180 GLMIRLSWHA AGTYRIHDGR GGAGDGGQRF APLNSWPDNV NLDKARRLLW PVKQKYGQKI 190 200 210 220 230 240 SWADLLVLAG NVALESMGFK TFGFGFGRED VWEPEEIFWG PEDTWLGDER YVSEKEFSAG 250 260 270 280 290 300 VGATEMGLIY VNPEGPRGNA DPASAAHFIR ETFRRMAMND EETVALIAGG HTFGKTHGAG 310 320 330 340 350 360 VADDHVGPEP EGAPLEAQGL GWMSSHASGV GADTISSGLE VTWTDRPTQW SNRFFEILFG 370 380 390 400 410 420 YEWELTTSPG GAKQWVAKDA EAIIPDAYDS TKKHKPTMLT TDLSLRVDPA YERISRRFLE 430 440 450 460 470 480 NPDEFALAFA KAWYKLLHRD MGPVSRFLGP WVPQTQLWQD PVPAVDHELV GAADIAALKA 490 500 510 520 530 540 KVLESGLTTT QLVSTAWASA ASFRHTDKRG GANGARIRLE PQRSWEVNQP EQLATVLPAL 550 560 570 580 590 600 EEIQREFNAA GGAKISLADL IVLAGSAAVE KAARDAGVEV TVPFRPGRTD ATQEQTDVDS 610 620 630 640 650 660 FRVLEPRADA FRNYLRPGEK TQPEVLLVDR AYLLNLTAPE MTVLIGGLRA LEANAGGSRH 670 680 690 700 710 720 GVLTDRPGVL TNDFFTNLLA SGARWKASES TEHAYEIRDV ATDKVKWTAS AVDLIFGSNS 730 740 750 QLRALAEVYA SEDAREKFVQ DFVAAWTKVM ELDRFDLA

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000667 Genomic DNA. Translation: ABP54507.1.
RefSeq	YP_001158885.1. NC_009380.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4X6K7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5058517.
GenomeReviews	Gene locus Strop_2054 in contig CP000667_GR.
KEGG	stp:Strop_2054.
PATRIC	23443130. VBISalTro43511_2107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	A4X6K7.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	STRO369723:STROP_2054-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SALTO

Accession

Primary (citable) accession number: A4X6K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program