Reviewed, UniProtKB/Swiss-Prot A4X639 (RIBBA_SALTO)

Names and origin

Protein names

Recommended name:
    Riboflavin biosynthesis protein ribBA
Including the following 2 domains:
    1- Recommended name:
            3,4-dihydroxy-2-butanone 4-phosphate synthase
                Short name=DHBP synthase
              EC=4.1.99.12
    2- Recommended name:
            GTP cyclohydrolase-2
              EC=3.5.4.25
        Alternative name(s):
            GTP cyclohydrolase II

Gene names

Name:	ribBA
Ordered Locus Names:	Strop_1877

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283 GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_01283
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283 Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283
Sequence similarities	In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Magnesium Manganese Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Riboflavin biosynthesis protein ribBA HAMAP MF_01283		PRO_1000085900
Regions
	Nucleotide binding	253 – 257	5	GTP By similarity
	Nucleotide binding	297 – 299	3	GTP By similarity
	Region	1 – 202	202	DHBP synthase HAMAP MF_01283
	Region	28 – 29	2	D-ribulose 5-phosphate binding By similarity
	Region	141 – 145	5	D-ribulose 5-phosphate binding By similarity
	Region	203 – 400	198	GTP cyclohydrolase II HAMAP MF_01283
Sites
	Active site	331	1	Proton acceptor; for GTP cyclohydrolase activity Potential
	Active site	333	1	Nucleophile; for GTP cyclohydrolase activity By similarity
	Metal binding	29	1	Magnesium or manganese 1 By similarity
	Metal binding	29	1	Magnesium or manganese 2 By similarity
	Metal binding	144	1	Magnesium or manganese 2 By similarity
	Metal binding	258	1	Zinc; catalytic By similarity
	Metal binding	269	1	Zinc; catalytic By similarity
	Metal binding	271	1	Zinc; catalytic By similarity
	Binding site	33	1	D-ribulose 5-phosphate By similarity
	Binding site	165	1	D-ribulose 5-phosphate By similarity
	Binding site	274	1	GTP By similarity
	Binding site	319	1	GTP By similarity
	Binding site	354	1	GTP By similarity
	Binding site	359	1	GTP By similarity
	Site	127	1	Essential for DHBP synthase activity By similarity
	Site	165	1	Essential for DHBP synthase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4X639-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: A81E77CCD9591AAF	FASTA	400	43,910
	10 20 30 40 50 60 MTTFGTIEQA MAEILAGRPV VVVDDANREN EGDLIFAAEL ATPELVAFMV RYTSGYICAS 70 80 90 100 110 120 LTEDDCDRLD LPPMHHTNQD RRGTAYMVTV DARKGVSTGI SAADRARVIR LLADPSTTPD 130 140 150 160 170 180 DLARPGHVVP LRAREGGVLR RTGHTEAATD LTRLAGLRPA GVLCELVNDD GTMMRVPDLE 190 200 210 220 230 240 RFCAEHSLVL VTIADLVMYR RRTEKQVELV AEARLPTRHG DFRVAGYRGD YDSAEHVALV 250 260 270 280 290 300 MGDLGDGRDV LVRAHSECLT GDVFGSLRCD CGLQLDTAME LVAKEGRGVV LYIRGHEGRG 310 320 330 340 350 360 IGLLHKLRAY QLQDKGRDTV EANLELGLPV DARDYGTGAQ VLYDLGVRSM RLLTNNPAKR 370 380 390 400 AGLEGYGLTV TERVALPVQS HPENERYLRT KRDRMGHLLG

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000667 Genomic DNA. Translation: ABP54339.1.
RefSeq	YP_001158717.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4X639.
Genome annotation databases
GeneID	5058336.
GenomeReviews	Gene locus Strop_1877 in contig CP000667_GR.
KEGG	stp:Strop_1877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HTEASLE.
Family and domain databases
HAMAP	MF_01283. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR000926. GTP_CycHdrlase_II. IPR016299. Riboflavin_synth_RibA. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
PIRSF	PIRSF001259. RibA. 1 hit.
ProDom	PD003034. DHBP_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00505. ribA. 1 hit. TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBBA_SALTO

Accession

Primary (citable) accession number: A4X639

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)