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Protein

Chorismate synthase

Gene

aroC

Organism
Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (Strop_3256), Phospho-2-dehydro-3-deoxyheptonate aldolase (Strop_1036), Phospho-2-dehydro-3-deoxyheptonate aldolase (Strop_3571)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401NADPUniRule annotation
Binding sitei46 – 461NADPUniRule annotation
Binding sitei300 – 3001FMN; via amide nitrogenUniRule annotation
Binding sitei341 – 3411FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 1373FMNUniRule annotation
Nucleotide bindingi256 – 2572FMNUniRule annotation
Nucleotide bindingi315 – 3195FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciSTRO369723:GI49-1875-MONOMER.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:Strop_1845
OrganismiSalinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)
Taxonomic identifieri369723 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeSalinispora
Proteomesi
  • UP000000235 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Chorismate synthasePRO_1000079008Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi369723.Strop_1845.

Structurei

3D structure databases

ProteinModelPortaliA4X607.
SMRiA4X607. Positions 1-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4X607-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRWLTAGES HGPALVALLE GVPAGVEVTT EEIAGELARR RLGYGRGARM
60 70 80 90 100
AFEQDEIEII GGLRHGVTLG SPVAIRVGNS EWPKWRTVMA PDPVDSTELA
110 120 130 140 150
GQARNAPLTR PRPGHADLAG MQKYGHTDAR PILERASARE TAARVAVGTV
160 170 180 190 200
AKALLRQALG IEVVSHVVEL GTVATKPGLR PSPADGDRID ADPLRCLDPE
210 220 230 240 250
ASVRMVAEVD AAKKDADTLG GVVEVLAYGV PPGLGSHVQW DRKLDARLAT
260 270 280 290 300
ALMSIQAIKG VEIGDGWQQA RSRGSVAHDE IIPTATGVRR VTDRAGGLEG
310 320 330 340 350
GITTGEPLRV RAAMKPISSL NRALSTVDIT SGEPATAINQ RSDVCAVPAA
360 370 380 390
GVVAEAMVAL VLAEAAVEKF GGDSVVEQRR NLAGYLDALV VR
Length:392
Mass (Da):41,154
Last modified:May 29, 2007 - v1
Checksum:i37562700E9E3D7C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000667 Genomic DNA. Translation: ABP54307.1.
RefSeqiWP_011905738.1. NC_009380.1.

Genome annotation databases

EnsemblBacteriaiABP54307; ABP54307; Strop_1845.
GeneIDi5058304.
KEGGistp:Strop_1845.
PATRICi23442694. VBISalTro43511_1893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000667 Genomic DNA. Translation: ABP54307.1.
RefSeqiWP_011905738.1. NC_009380.1.

3D structure databases

ProteinModelPortaliA4X607.
SMRiA4X607. Positions 1-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi369723.Strop_1845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP54307; ABP54307; Strop_1845.
GeneIDi5058304.
KEGGistp:Strop_1845.
PATRICi23442694. VBISalTro43511_1893.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciSTRO369723:GI49-1875-MONOMER.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Salinispora tropica CNB-440."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Entry informationi

Entry nameiAROC_SALTO
AccessioniPrimary (citable) accession number: A4X607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: November 11, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.