A4X5Z0 (SYA_SALTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Strop_1828

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347776
Sites
	Metal binding	580	1	Zinc Potential
	Metal binding	584	1	Zinc Potential
	Metal binding	682	1	Zinc Potential
	Metal binding	686	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A4X5Z0 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 9A52DEBDA6EC954B	FASTA	892	96,214
	10 20 30 40 50 60 MKTAEIRRRY LAHFEANGHA VVPSAPLPAI SDPNLLFVAA GMMQFVPYFL GQQTAPYKRA 70 80 90 100 110 120 VSVQKCLRTP DIDEVGKTSR HGTFFQMNGN FSFGDYFKDE AIPLAWELST KPVDAGGLGL 130 140 150 160 170 180 DPDRIWPTVY LDDDEAFQIW RSVGVPADRI VRRGKADNFW SMGIPGPCGP CSELFYDRGP 190 200 210 220 230 240 EYGREGGPEV DEDRYLEFWN LVFMQFERGP GTGKEDYPIL GDLPAKNIDT GMGLERMASI 250 260 270 280 290 300 LQGVDNLYEI DEVRPILAKA AELTGKRYGA HSGQVASESH PDDVRLRVVA DHVRTALMLI 310 320 330 340 350 360 GDGVIPSNEG RGYVLRRIMR RAIRSIRLLG WQERAMPELL PVARDCMSAS YPELATDFGR 370 380 390 400 410 420 IADYAYAEED AFLSTLRAGT TILDTAIAET RTAGRRAISG AKAFQLHDTY GFPIDLTLEI 430 440 450 460 470 480 ASEQGLQVDA EGFRRLMADQ RARAKADAQA RKTGHVDLSA YRTVLDEGGP VTFTGYQEVS 490 500 510 520 530 540 RESTVRAVLG AASPHAAAVE GETVELVLDT TPFYAEGGGQ QPDLGVITVG GGQVEVLDVQ 550 560 570 580 590 600 QPVPGLIVHR ARVLRGEVRV GETGFAEIDT DRRRAISRSH TATHLVHQTM RNFLGESATQ 610 620 630 640 650 660 AGSLNAPGRL RFDFNTPTGV APSVLRDVEQ QVNEALLADL EVRAFVTSLA EARRIGAMAL 670 680 690 700 710 720 FGEKYGEQVR VVEVGDYARE LCGGTHVGRS GQLGLVKILS ESSIGSGVRR VEALVGIDAF 730 740 750 760 770 780 NFLAREHLLV ARLAELYRVP SDQVAERVEQ TVTQLRDAEK ELEKLRAQLV LGGAAALAAQ 790 800 810 820 830 840 ASEVRGVAYV GTEAPEGAAG NDVRTLAQEI RSRIDPARPA VVAVAARANG KASLVVAVNP 850 860 870 880 890 AARSQGLSAA DLVKVAFAGR GGGSPELAQG GGLPAAEAPG LLRTVENAIA GA

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000667 Genomic DNA. Translation: ABP54290.1.
RefSeq	YP_001158668.1. NC_009380.1.
3D structure databases
ProteinModelPortal	A4X5Z0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4X5Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5058287.
GenomeReviews	Gene locus Strop_1828 in contig CP000667_GR.
KEGG	stp:Strop_1828.
PATRIC	23442660. VBISalTro43511_1876.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	GESKTDQ.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	STRO369723:STROP_1828-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_SALTO

Accession

Primary (citable) accession number: A4X5Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program