A4X5V6 (SYP_SALTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Strop_1793

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Proline--tRNA ligase HAMAP MF_01569		PRO_1000087852

Sequences

	Sequence		Length	Mass (Da)
	A4X5V6 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 9E4FEDD6DA9F7F23	FASTA	595	64,340
	10 20 30 40 50 60 MKMSTMFGAT LHTAPGRSES EGHQLLQRAA MLRQVGQGIF AYLPLGWRTI RKIEAVLRAE 70 80 90 100 110 120 LERVGGQEVS MPVVNPAELW KQTGRYFTIG PELARFRDRR GRDLVLAMTH EELVTFLGRS 130 140 150 160 170 180 EIESYRHLPR MVFQLQTKFR DDPRPRAGLI RVREFVMKDA YSFDVDAAGL AARYRAQYQA 190 200 210 220 230 240 YLTIFRRCGL PVAAVLSDVG MMGGSLAHEF VYLTPIGEDT LLICDSCGFA ANREAATFGK 250 260 270 280 290 300 PAPSTAPWAE LAEIATPGAT TIEALTAALG VPAAETAKAI FLAASREDRD QTVDTEFILA 310 320 330 340 350 360 VVRGDMEANE TKIGNLVQAA ELRPMTVEEI SKIGAVAGYG SPVGVTGVTV VVDELVAAST 370 380 390 400 410 420 NLVAGANREG MHLRNVNVGR DFVADHTGDI TAARAGDACA ECGSELRTAR GVEVGQIFKL 430 440 450 460 470 480 GTRYSTALGA EYLDADGQRK PLTMGCYGIG VGRLLACLAE EHRDERGLRL PVTIAPYQVH 490 500 510 520 530 540 LTLLDDLASA AGELARRVYD ELWAAGVEVL FDDRDERAGV KFADADVIGL PLRVTIGRRS 550 560 570 580 590 VANGAAEVRD RATGVTAQVP FDEVVAELVR RIAALRADID ATVVPVELPA EVFAP

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000667 Genomic DNA. Translation: ABP54256.1.
RefSeq	YP_001158634.1. NC_009380.1.
3D structure databases
ProteinModelPortal	A4X5V6.
ModBase	Search...
Protein-protein interaction databases
STRING	A4X5V6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5058252.
GenomeReviews	Gene locus Strop_1793 in contig CP000667_GR.
KEGG	stp:Strop_1793.
PATRIC	23442588. VBISalTro43511_1840.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	IQPAELW.
PhylomeDB	A4X5V6.
Enzyme and pathway databases
BioCyc	STRO369723:STROP_1793-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_SALTO

Accession

Primary (citable) accession number: A4X5V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program