A4X4I2 (RNH2_SALTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	Strop_1312

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Ribonuclease HII HAMAP MF_00052_B		PRO_0000334953
Sites
	Metal binding	34	1	Divalent metal cation By similarity
	Metal binding	35	1	Divalent metal cation By similarity
	Metal binding	128	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4X4I2 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: C856FF8C0ED4F5B0	FASTA	270	28,273
	10 20 30 40 50 60 MLTPPRTVVR RDGGLYALER ALQRRGFRQV AGADEAGRGA CAGPLVAAAA ILPPGRRGEV 70 80 90 100 110 120 DGLADSKLLT PASRERVYAE VIARASAYAV VVIPAAEVDL RGLHVCNLAA MRRALASLAT 130 140 150 160 170 180 PPEYVLTDGF GVDGLDVPGL AVWKGDRVAA CVAAASVLAK VTRDRIMVEL DLEFPGYGFA 190 200 210 220 230 240 EHKGYITAEH SAALRERGPC PEHRFSYVNV ATVSGRQGAP PRARRPLVAE ADEAMERAGV 250 260 270 VKGTVGVALG ERPWAGASVG NDVAMEGGMG

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000667 Genomic DNA. Translation: ABP53782.1.
RefSeq	YP_001158160.1. NC_009380.1.
3D structure databases
ProteinModelPortal	A4X4I2.
ModBase	Search...
Protein-protein interaction databases
STRING	A4X4I2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5057765.
GenomeReviews	Gene locus Strop_1312 in contig CP000667_GR.
KEGG	stp:Strop_1312.
PATRIC	23441570. VBISalTro43511_1337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
HOGENOM	HBG584843.
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	STRO369723:STROP_1312-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_SALTO

Accession

Primary (citable) accession number: A4X4I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program