A4X4A1 (A4X4A1_SALTO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 2 HAMAP MF_00012
Short name=DAD 2 HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD2 HAMAP MF_00012
Ordered Locus Names:	Strop_1231

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP] EMBL ABP53701.1

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL ABP53701.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	145	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	220	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	A4X4A1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 32CCF7640DF1380B	FASTA	638	67,078
	10 20 30 40 50 60 MCICWDTSCA GSGRTRDGVR LVCMPELRSR TSTHGRTMAG ARALWRATGM TDDDFGKPIV 70 80 90 100 110 120 AIANSFTQFV PGHVHLKELG GLVAEAVADS GGVGREFNTI AVDDGIAMGH GGMLYSLPSR 130 140 150 160 170 180 ELIADAVEYM VNAHCADALV CISNCDKITP GMLLAALRLN IPTVFVSGGP MEAGKTVAIE 190 200 210 220 230 240 GVVHSKIDLI DAMIAASNEA VTDDQLGQIE RSACPTCGSC SGMFTANSMN CLTEAIGLAL 250 260 270 280 290 300 PGNGSTLATH AARRSLFVEA GRTVVEIAKR WYDGDDATVL PRAVANRAAF DNAVALDVAM 310 320 330 340 350 360 GGSTNTILHL LAAAREAELD FGVVDIDAIS RRVPCLAKVA PNSPHYHMED VHRAGGIPAI 370 380 390 400 410 420 LGELDRAGLL NREVHAVHSP SLERWLADWD VRGGTATPTA VELFHAAPGG VRTVEPFSTT 430 440 450 460 470 480 NRWSTLDTDA ADGCVRERAH AYTADGGLAI LHGNLAPEGC VVKTAGVPEE CLTFRGPAKV 490 500 510 520 530 540 YESQDDAVTA ILAKEVVAGD VVVIRYEGPR GGPGMQEMLY PTSFLKGRGL GRACALLTDG 550 560 570 580 590 600 RFSGGTSGLS VGHVSPEAAA GGLIALVEPG DEIVIDIPNR AIELAVPAEV LDARRVAQEK 610 620 630 RDRPYTPAER QRPVSAALRA YAAMTTSASD GAYRRVPE

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000667 Genomic DNA. Translation: ABP53701.1.
RefSeq	YP_001158079.1. NC_009380.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4X4A1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5057681.
GenomeReviews	Gene locus Strop_1231 in contig CP000667_GR.
KEGG	stp:Strop_1231.
PATRIC	23441398. VBISalTro43511_1254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	A4X4A1.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	STRO369723:STROP_1231-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4X4A1_SALTO

Accession

Primary (citable) accession number: A4X4A1

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)