A4X491 (GATB_SALTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	Strop_1221

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora › Salinispora tropica

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_1000095242

Sequences

	Sequence		Length	Mass (Da)
	A4X491 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 2DCB1A5CAB3130F7	FASTA	499	53,726
	10 20 30 40 50 60 MTTTLPAYDE VVARYEPVIG LETHVELGTN TKMFCGCPTD FGGAPNTRVC PVCLGLPGSL 70 80 90 100 110 120 PVANRAAVEA TIRIGLALNC SIAEWCRFAR KNYYYPDMPK NYQISQYDEP LCVDGYLDVE 130 140 150 160 170 180 VDGEPVRISI ERVHMEEDTG KTLHVGGATG RIHGATESLV DYNRAGIPLV EIVTKPIPGA 190 200 210 220 230 240 GAMAPEVARA YVTELRDVLR SLGVSDVRME EGSLRCDVNT SLNLPGEQWG TRTETKNVNS 250 260 270 280 290 300 LRSVERAVRS EMIRQASVLE GGGRITQETR HFHEDTGDTT SGRSKETATD YRYFPEPDLV 310 320 330 340 350 360 PVAPDPTWVA ELKAALPELP RLHRRRLQQE WGLSDLDMQS ILNAGAVELI EATIAAGATP 370 380 390 400 410 420 TAARKWWLGE LSRRANEAGV ELADIGATPE QVAELQGLVD AGKLTDKLAR TVLEHVVAGE 430 440 450 460 470 480 GSPAKIMADR NLEVVSDTGA LTAAVDEAIA ANPAIADKVR GGKVAAAGAL VGAVMKTTRG 490 QADAKTVREL ILERLGVQG

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000667 Genomic DNA. Translation: ABP53691.1.
RefSeq	YP_001158069.1. NC_009380.1.
3D structure databases
ProteinModelPortal	A4X491.
ModBase	Search...
Protein-protein interaction databases
STRING	369723.Strop_1221.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABP53691; ABP53691; Strop_1221.
GeneID	5057671.
KEGG	stp:Strop_1221.
PATRIC	23441378. VBISalTro43511_1244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223742.
KO	K02434.
OMA	GWVEIEV.
ProtClustDB	PRK05477.
Family and domain databases
Gene3D	1.10.10.410. 1 hit.
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_SALTO

Accession

Primary (citable) accession number: A4X491

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 29, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program