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A4X388

- PCKG_SALTO

UniProt

A4X388 - PCKG_SALTO

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei82 – 821SubstrateUniRule annotation
    Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
    Metal bindingi230 – 2301ManganeseUniRule annotation
    Binding sitei230 – 2301SubstrateUniRule annotation
    Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Active sitei274 – 2741UniRule annotation
    Metal bindingi297 – 2971ManganeseUniRule annotation
    Binding sitei389 – 3891GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi273 – 2786GTPUniRule annotation
    Nucleotide bindingi517 – 5204GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSTRO369723:GI49-875-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:Strop_0861
    OrganismiSalinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)
    Taxonomic identifieri369723 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeSalinispora
    ProteomesiUP000000235: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_1000080989Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi369723.Strop_0861.

    Structurei

    3D structure databases

    ProteinModelPortaliA4X388.
    SMRiA4X388. Positions 13-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 3893Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiINTEGLG.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4X388-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAPATVRGI DQAPTSHPKL LAWVREIAEL TTPDRVVWAD GSEEEWRRIT    50
    DELVEAGTLV RLNPEKKRNS FYARTDPTDV ARVEERTFIC SVDEADAGPT 100
    NNWMAPAEMK RTMTELYRGC MRGRTMYVIP FCMGPVEAKN PMFGIEITDS 150
    PYVVASMRIM TRMGTKILEA MGDDADFVHA LHSIGAPLAP GQQDVSWPCN 200
    ETKYISHFPE TREIWSYGSG YGGNSLLGKK CYSLRIASVM GRDEGWLAEH 250
    MLILKITSPE GRAYHIAGAF PSACGKTNLA MLEPTIPGWK VETVGDDIAW 300
    MRFGPDGRLY AVNPEYGLFG VAPGTDWKTN ANAMRTLDRG NSIFTNVALT 350
    DDGDIWWEGM GEPPTHLIDW KGNDWTPQSE HLSSHANSRF CTPITQCPIL 400
    AEDYYDPNGV PIDAILFGGR RRDTVPLVTE ARDWVHGVYL GATLSSETTA 450
    AASGAVGVVR RDPMAMLPFI GYNAGDYFRH WIEMGKGTDG DESKLPSVYY 500
    VNWFRKDAEG SFLWPGFGEN SRVLKWIVER LEGRAEAVET PIGMVPAEDA 550
    LDVEGLDMTS EDIRIALKVD VNEWQAELPL VTEWFEKFGD KLPGVLWAEL 600
    DALRARLDAE PQ 612
    Length:612
    Mass (Da):68,188
    Last modified:May 29, 2007 - v1
    Checksum:i6E8EF4A30279CA17
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000667 Genomic DNA. Translation: ABP53338.1.
    RefSeqiYP_001157716.1. NC_009380.1.

    Genome annotation databases

    EnsemblBacteriaiABP53338; ABP53338; Strop_0861.
    GeneIDi5057304.
    KEGGistp:Strop_0861.
    PATRICi23440635. VBISalTro43511_0879.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000667 Genomic DNA. Translation: ABP53338.1 .
    RefSeqi YP_001157716.1. NC_009380.1.

    3D structure databases

    ProteinModelPortali A4X388.
    SMRi A4X388. Positions 13-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 369723.Strop_0861.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP53338 ; ABP53338 ; Strop_0861 .
    GeneIDi 5057304.
    KEGGi stp:Strop_0861.
    PATRICi 23440635. VBISalTro43511_0879.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi INTEGLG.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci STRO369723:GI49-875-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Salinispora tropica CNB-440."
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P.
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

    Entry informationi

    Entry nameiPCKG_SALTO
    AccessioniPrimary (citable) accession number: A4X388
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3