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A4X388 (PCKG_SALTO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:Strop_0861
OrganismSalinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]
Taxonomic identifier369723 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeSalinispora

Protein attributes

Sequence length612 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 612612Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000080989

Regions

Nucleotide binding273 – 2786GTP By similarity
Nucleotide binding517 – 5204GTP By similarity
Region387 – 3893Substrate binding By similarity

Sites

Active site2741 By similarity
Metal binding2301Manganese By similarity
Metal binding2501Manganese; via tele nitrogen By similarity
Metal binding2971Manganese By similarity
Binding site821Substrate By similarity
Binding site2231Substrate; via amide nitrogen By similarity
Binding site2301Substrate By similarity
Binding site2721Substrate By similarity
Binding site3891GTP By similarity
Binding site4201GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4X388 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 6E8EF4A30279CA17

FASTA61268,188
        10         20         30         40         50         60 
MVAPATVRGI DQAPTSHPKL LAWVREIAEL TTPDRVVWAD GSEEEWRRIT DELVEAGTLV 

        70         80         90        100        110        120 
RLNPEKKRNS FYARTDPTDV ARVEERTFIC SVDEADAGPT NNWMAPAEMK RTMTELYRGC 

       130        140        150        160        170        180 
MRGRTMYVIP FCMGPVEAKN PMFGIEITDS PYVVASMRIM TRMGTKILEA MGDDADFVHA 

       190        200        210        220        230        240 
LHSIGAPLAP GQQDVSWPCN ETKYISHFPE TREIWSYGSG YGGNSLLGKK CYSLRIASVM 

       250        260        270        280        290        300 
GRDEGWLAEH MLILKITSPE GRAYHIAGAF PSACGKTNLA MLEPTIPGWK VETVGDDIAW 

       310        320        330        340        350        360 
MRFGPDGRLY AVNPEYGLFG VAPGTDWKTN ANAMRTLDRG NSIFTNVALT DDGDIWWEGM 

       370        380        390        400        410        420 
GEPPTHLIDW KGNDWTPQSE HLSSHANSRF CTPITQCPIL AEDYYDPNGV PIDAILFGGR 

       430        440        450        460        470        480 
RRDTVPLVTE ARDWVHGVYL GATLSSETTA AASGAVGVVR RDPMAMLPFI GYNAGDYFRH 

       490        500        510        520        530        540 
WIEMGKGTDG DESKLPSVYY VNWFRKDAEG SFLWPGFGEN SRVLKWIVER LEGRAEAVET 

       550        560        570        580        590        600 
PIGMVPAEDA LDVEGLDMTS EDIRIALKVD VNEWQAELPL VTEWFEKFGD KLPGVLWAEL 

       610 
DALRARLDAE PQ 

« Hide

References

[1]"Complete sequence of Salinispora tropica CNB-440."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000667 Genomic DNA. Translation: ABP53338.1.
RefSeqYP_001157716.1. NC_009380.1.

3D structure databases

ProteinModelPortalA4X388.
SMRA4X388. Positions 13-608.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING369723.Strop_0861.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP53338; ABP53338; Strop_0861.
GeneID5057304.
KEGGstp:Strop_0861.
PATRIC23440635. VBISalTro43511_0879.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAINTEGLG.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

BioCycSTRO369723:GI49-875-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_SALTO
AccessionPrimary (citable) accession number: A4X388
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways