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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82SubstrateUniRule annotation1
Metal bindingi230ManganeseUniRule annotation1
Metal bindingi250Manganese; via tele nitrogenUniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Active sitei274UniRule annotation1
Metal bindingi297ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 278GTPUniRule annotation6
Nucleotide bindingi517 – 520GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Strop_0861
OrganismiSalinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)
Taxonomic identifieri369723 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeSalinispora
Proteomesi
  • UP000000235 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000809891 – 612Phosphoenolpyruvate carboxykinase [GTP]Add BLAST612

Proteomic databases

PRIDEiA4X388.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi369723.Strop_0861.

Structurei

3D structure databases

ProteinModelPortaliA4X388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 223Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4X388-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAPATVRGI DQAPTSHPKL LAWVREIAEL TTPDRVVWAD GSEEEWRRIT
60 70 80 90 100
DELVEAGTLV RLNPEKKRNS FYARTDPTDV ARVEERTFIC SVDEADAGPT
110 120 130 140 150
NNWMAPAEMK RTMTELYRGC MRGRTMYVIP FCMGPVEAKN PMFGIEITDS
160 170 180 190 200
PYVVASMRIM TRMGTKILEA MGDDADFVHA LHSIGAPLAP GQQDVSWPCN
210 220 230 240 250
ETKYISHFPE TREIWSYGSG YGGNSLLGKK CYSLRIASVM GRDEGWLAEH
260 270 280 290 300
MLILKITSPE GRAYHIAGAF PSACGKTNLA MLEPTIPGWK VETVGDDIAW
310 320 330 340 350
MRFGPDGRLY AVNPEYGLFG VAPGTDWKTN ANAMRTLDRG NSIFTNVALT
360 370 380 390 400
DDGDIWWEGM GEPPTHLIDW KGNDWTPQSE HLSSHANSRF CTPITQCPIL
410 420 430 440 450
AEDYYDPNGV PIDAILFGGR RRDTVPLVTE ARDWVHGVYL GATLSSETTA
460 470 480 490 500
AASGAVGVVR RDPMAMLPFI GYNAGDYFRH WIEMGKGTDG DESKLPSVYY
510 520 530 540 550
VNWFRKDAEG SFLWPGFGEN SRVLKWIVER LEGRAEAVET PIGMVPAEDA
560 570 580 590 600
LDVEGLDMTS EDIRIALKVD VNEWQAELPL VTEWFEKFGD KLPGVLWAEL
610
DALRARLDAE PQ
Length:612
Mass (Da):68,188
Last modified:May 29, 2007 - v1
Checksum:i6E8EF4A30279CA17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000667 Genomic DNA. Translation: ABP53338.1.
RefSeqiWP_011904772.1. NC_009380.1.

Genome annotation databases

EnsemblBacteriaiABP53338; ABP53338; Strop_0861.
GeneIDi5057304.
KEGGistp:Strop_0861.
PATRICi23440635. VBISalTro43511_0879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000667 Genomic DNA. Translation: ABP53338.1.
RefSeqiWP_011904772.1. NC_009380.1.

3D structure databases

ProteinModelPortaliA4X388.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi369723.Strop_0861.

Proteomic databases

PRIDEiA4X388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP53338; ABP53338; Strop_0861.
GeneIDi5057304.
KEGGistp:Strop_0861.
PATRICi23440635. VBISalTro43511_0879.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_SALTO
AccessioniPrimary (citable) accession number: A4X388
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.