A4X310 (ISPE_SALTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Strop_0783

Organism

Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) [Complete proteome] [HAMAP]

Taxonomic identifier

369723 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Salinispora

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335754
Regions
	Nucleotide binding	111 – 121	11	ATP Potential
Sites
	Active site	26	1	By similarity
	Active site	153	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4X310 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: E02EECF67ABB024F	FASTA	315	31,943
	10 20 30 40 50 60 MTEAWRPEDD EPRGASGPVR VRVPAKINLH LGVGPLRRDG YHELNTVYHA ISIHDELTAR 70 80 90 100 110 120 RGDTLTLTME GEGAGELALD ESNLVIRAAR ALAGSTGVPP HARLHLRKQI PLAGGLAGGS 130 140 150 160 170 180 ADAAAALVAC DALWGTGLTR DELAEIAAGL GSDVPFLIHG GTALGTGRGE AVSPVLARPT 190 200 210 220 230 240 VWHWVVAVAD GGLSTPVAYR ELDRLRAAGA AGPPLGSTDT LLAALRQPDP RVLAAALGND 250 260 270 280 290 300 LQDAALALRP ALAATLKAGE AAGALAGIVS GSGPTCVFLA AGAADAERIA AELSALDVCR 310 QARTAHGPVA GARIG

References

[1]

"Complete sequence of Salinispora tropica CNB-440." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sun H., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Jensen P.R., Moore B.S., Udwary D.W., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-916 / DSM 44818 / CNB-440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000667 Genomic DNA. Translation: ABP53260.1.
RefSeq	YP_001157638.1. NC_009380.1.
3D structure databases
ProteinModelPortal	A4X310.
ModBase	Search...
Protein-protein interaction databases
STRING	A4X310.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5057225.
GenomeReviews	Gene locus Strop_0783 in contig CP000667_GR.
KEGG	stp:Strop_0783.
PATRIC	23440463. VBISalTro43511_0794.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	KINLHLG.
PhylomeDB	A4X310.
ProtClustDB	PRK03188.
Enzyme and pathway databases
BioCyc	STRO369723:STROP_0783-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SALTO

Accession

Primary (citable) accession number: A4X310

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program