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Protein

Catalase-peroxidase

Gene

katG

Organism
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei95 – 951Transition state stabilizerUniRule annotation
Active sitei99 – 991Proton acceptorUniRule annotation
Metal bindingi267 – 2671Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciRSPH349102:GHE1-620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:Rsph17025_3306
Encoded oniPlasmid pRSPA010 Publication
OrganismiRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Taxonomic identifieri349102 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000000234 Componenti: Plasmid pRSPA01

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724Catalase-peroxidasePRO_0000354889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki98 ↔ 226Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252)UniRule annotation
Cross-linki226 ↔ 252Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4WXS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGNGIGSTG KCPVMHGGNT AMGTSNTDWW PNALNLDILH QHDSKTNPMG
60 70 80 90 100
PGFDYRRALQ GLDVTALKRD LHALMTDSQD WWPADWGHYG GLMIRMAWHA
110 120 130 140 150
AGSYRTADGR GGGGTGNQRF APLNSWPDNV NLDKARRLLW PVKKKYGNAI
160 170 180 190 200
SWADLIILAG TVAYESMGLK TFGFAFGRAD IWHPEKDTYW GAEKEWLAPS
210 220 230 240 250
DSRYGDVADP ASMENPLAAV QMGLIYVNPQ GVNGEPDPLR TALHVRETFA
260 270 280 290 300
RMAMNDEETV ALTAGGHTVG KCHGNGDAAL IGPDPEAADV VEQGLGWMNH
310 320 330 340 350
STRGVGRNTV TSGIEGAWTT HPTRWDNGYF DLLFGYEWEL RKSPAGAWQW
360 370 380 390 400
EPIDIREEDK PVDVEDPSIR YNPIMTDADM AMKMDPIYRP IAERFHRDPA
410 420 430 440 450
YFSDVFARAW FKLTHRDMGP KARYLGPDVP QEDLIWQDPV PAGRTDYDVA
460 470 480 490 500
AVKARIAASG LSVAELVSTA WDSARTFRGS DMRGGANGAR IRLAPQKDWE
510 520 530 540 550
GNEPARLATV LGVLEGIAAE TGASVADVIV LGGNVGVEQA ARAAGFEITV
560 570 580 590 600
PFAPGRGDAT DDMTDAASFD VLEPIHDAFR NWLKKDYTVS PEELMLDRAQ
610 620 630 640 650
LMGLTAHEMT VLLGGMRVLG TNHGGTKHGV LTDREGALTT DFFVNLTDMA
660 670 680 690 700
NVWKPADANL YEIRDRRTGA VKWTATRVDL VFGSNSVLRA YAEVYAQDDS
710 720
REKFVQDFVA AWAKVMNADR FDIA
Length:724
Mass (Da):79,546
Last modified:May 29, 2007 - v1
Checksum:i4B461C65AB06C722
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000662 Genomic DNA. Translation: ABP72190.1.
RefSeqiWP_011910249.1. NC_009429.1.

Genome annotation databases

EnsemblBacteriaiABP72190; ABP72190; Rsph17025_3306.
KEGGirsq:Rsph17025_3306.
PATRICi23165057. VBIRhoSph94549_3428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000662 Genomic DNA. Translation: ABP72190.1.
RefSeqiWP_011910249.1. NC_009429.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP72190; ABP72190; Rsph17025_3306.
KEGGirsq:Rsph17025_3306.
PATRICi23165057. VBIRhoSph94549_3428.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiIAEVYAC.
OrthoDBiPOG091H05R1.

Enzyme and pathway databases

BioCyciRSPH349102:GHE1-620-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_RHOS5
AccessioniPrimary (citable) accession number: A4WXS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.