A4WXS6 (KATG_RHOS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Rsph17025_3306

Encoded on

Plasmid pRSPA01

Organism

Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) [Complete proteome] [HAMAP]

Taxonomic identifier

349102 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Catalase-peroxidase HAMAP MF_01961		PRO_0000354889
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WXS6 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 4B461C65AB06C722	FASTA	724	79,546
	10 20 30 40 50 60 MDGNGIGSTG KCPVMHGGNT AMGTSNTDWW PNALNLDILH QHDSKTNPMG PGFDYRRALQ 70 80 90 100 110 120 GLDVTALKRD LHALMTDSQD WWPADWGHYG GLMIRMAWHA AGSYRTADGR GGGGTGNQRF 130 140 150 160 170 180 APLNSWPDNV NLDKARRLLW PVKKKYGNAI SWADLIILAG TVAYESMGLK TFGFAFGRAD 190 200 210 220 230 240 IWHPEKDTYW GAEKEWLAPS DSRYGDVADP ASMENPLAAV QMGLIYVNPQ GVNGEPDPLR 250 260 270 280 290 300 TALHVRETFA RMAMNDEETV ALTAGGHTVG KCHGNGDAAL IGPDPEAADV VEQGLGWMNH 310 320 330 340 350 360 STRGVGRNTV TSGIEGAWTT HPTRWDNGYF DLLFGYEWEL RKSPAGAWQW EPIDIREEDK 370 380 390 400 410 420 PVDVEDPSIR YNPIMTDADM AMKMDPIYRP IAERFHRDPA YFSDVFARAW FKLTHRDMGP 430 440 450 460 470 480 KARYLGPDVP QEDLIWQDPV PAGRTDYDVA AVKARIAASG LSVAELVSTA WDSARTFRGS 490 500 510 520 530 540 DMRGGANGAR IRLAPQKDWE GNEPARLATV LGVLEGIAAE TGASVADVIV LGGNVGVEQA 550 560 570 580 590 600 ARAAGFEITV PFAPGRGDAT DDMTDAASFD VLEPIHDAFR NWLKKDYTVS PEELMLDRAQ 610 620 630 640 650 660 LMGLTAHEMT VLLGGMRVLG TNHGGTKHGV LTDREGALTT DFFVNLTDMA NVWKPADANL 670 680 690 700 710 720 YEIRDRRTGA VKWTATRVDL VFGSNSVLRA YAEVYAQDDS REKFVQDFVA AWAKVMNADR FDIA

References

[1]

"Complete sequence of plasmid pRSPA01 of Rhodobacter sphaeroides ATCC 17025." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17025 / ATH 2.4.3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000662 Genomic DNA. Translation: ABP72190.1.
RefSeq	YP_001169495.1. NC_009429.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4WXS6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5085797.
GenomeReviews	Gene locus Rsph17025_3306 in contig CP000662_GR.
KEGG	rsq:Rsph17025_3306.
PATRIC	23165057. VBIRhoSph94549_3428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	RSPH349102:RSPH17025_3306-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_RHOS5

Accession

Primary (citable) accession number: A4WXS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program