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A4WXA5 (SYL_RHOS5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Rsph17025_3135
OrganismRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) [Complete proteome] [HAMAP]
Taxonomic identifier349102 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 873873Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009415

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif645 – 6495"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6481ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4WXA5 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 9625CE7AD78EB108

FASTA87397,366
        10         20         30         40         50         60 
MSRYDPAATE SRWQAAWDEA GVFTARHDPS RPKYYVLEMF PYPSGRIHMG HVRNYTMGDV 

        70         80         90        100        110        120 
VARQKAAAGY SVLHPMGWDA FGMPAENAAM ERGGHPKDWT YGNIADMRAQ MKPLGLSIDW 

       130        140        150        160        170        180 
SREFATCDPE YYGQQQAMFI DMLEAGLIYR KNAVVNWDPV DMTVLANEQV IDGKGWRSNA 

       190        200        210        220        230        240 
PVERRELTQW FFRISDYAGE LLEALDRLKD WPEKVRLMQA NWIGQSRGLQ FAFSTVNAPE 

       250        260        270        280        290        300 
GFDRLEVYTT RPDTLLGASF AAISPDHPLA KHLERHDAGV AEFVAECRRV GTSEEALEKA 

       310        320        330        340        350        360 
EKKGFDTGIR VRHPFDTALE LPVYIANFIL MDYGTGAIFG CPAHDQRDFE FASKYGLPIP 

       370        380        390        400        410        420 
PVFVAEGTEE APLTEAFVPM KSERVAYVRG FAGAEIQTGE EAVAAAIDFC ESKGVGRGVT 

       430        440        450        460        470        480 
NYRLRDWGIS RQRYWGCPIP VIHCETCGVV PEAKENLPVR LPDDVTFDVP GNPLDRHPTW 

       490        500        510        520        530        540 
RDCTCPKCGA KARRETDTMD TFVDSSWYYA RFTAPRATTP TDPEEAEYWM NVDQYIGGIE 

       550        560        570        580        590        600 
HAILHLLYSR FFARAMQKTG HLPAKAIEPF NALFTQGMVT HEAYYSEEAK QEPVWQGMLE 

       610        620        630        640        650        660 
PGESPDTQMT VRHITYHFPE EVERTEDGAI LKTTGQKLKV IPSTKMSKSK KNVVDPMNII 

       670        680        690        700        710        720 
SQFGADTARW FVMSDSPPER DVEWTASGAE AAFKHLGRVW RLADEIARAE GAPTAEDVAL 

       730        740        750        760        770        780 
DRATAKAIAE VTQGIEGFAF NKAIAKLYEF TNTLSRSGAG AEAKRRAMRT MAQLMSPMVP 

       790        800        810        820        830        840 
HLAEEVWSML GGEGLVAQAP WPKADPALLV DDMVTLPIQI NGKRRAEVTV PKDMAASEVE 

       850        860        870 
KLVLADEAVQ RALSGGAPKK LIVVPGRIVN VVI 

« Hide

References

[1]"Complete sequence of chromosome of Rhodobacter sphaeroides ATCC 17025."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17025 / ATH 2.4.3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000661 Genomic DNA. Translation: ABP72019.1.
RefSeqYP_001169324.1. NC_009428.1.

3D structure databases

ProteinModelPortalA4WXA5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349102.Rsph17025_3135.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP72019; ABP72019; Rsph17025_3135.
GeneID5083087.
KEGGrsq:Rsph17025_3135.
PATRIC23164696. VBIRhoSph94549_3248.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycRSPH349102:GHE1-4461-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RHOS5
AccessionPrimary (citable) accession number: A4WXA5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries