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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei246ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSPH349102:G1G8M-3195-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:Rsph17025_3092
OrganismiRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Taxonomic identifieri349102 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000000234 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677461 – 471Glutamate--tRNA ligase 2Add BLAST471

Proteomic databases

PRIDEiA4WX62

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349102.Rsph17025_3092

Structurei

3D structure databases

ProteinModelPortaliA4WX62
SMRiA4WX62
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi15 – 25"HIGH" regionUniRule annotationAdd BLAST11
Motifi243 – 247"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A4WX62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAASEKPVV TRFAPSPTGY LHIGGARTAL FNWLFARGRK GTFLLRIEDT
60 70 80 90 100
DRERSTPEAT DAILRGLTWL GLDWDGEVVS QFARKDRHAE VAREMLARGA
110 120 130 140 150
AYKCFSTQDE IEAFREAARA EGRSTLFRSP WREADPSTHP DAPYVIRMKA
160 170 180 190 200
PRTGETVIAD RVQGTVRFQN ETLDDMVVLR SDGTPTYMLA VVVDDHDMGV
210 220 230 240 250
THVIRGDDHL NNAARQTMVY EAMGWEVPIW AHIPLIHGPD GKKLSKRHGA
260 270 280 290 300
LGVEEYQAMG YPAAGMRNYL TRLGWAHGDD EFFTSEQAMA WFDLEGIGRS
310 320 330 340 350
PARLDFKKLE NVCGQHIAVM DDAAAMHEIQ AYLAAARKPA LTDLQAGRLS
360 370 380 390 400
AALYALKDRA KTFPELLEKA RFALESRPIA ADDAAAKALD AVSRGILREL
410 420 430 440 450
TPILQTASWS KQELEAALTA FAGEKGMGFG KLAGPLRAAL AGRTVTPSVF
460 470
DMMLVIGRDE TIARLEDAAA A
Length:471
Mass (Da):51,811
Last modified:May 29, 2007 - v1
Checksum:iD23D3554E79E7DCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000661 Genomic DNA Translation: ABP71976.1
RefSeqiWP_011910035.1, NC_009428.1

Genome annotation databases

EnsemblBacteriaiABP71976; ABP71976; Rsph17025_3092
KEGGirsq:Rsph17025_3092

Similar proteinsi

Entry informationi

Entry nameiSYE2_RHOS5
AccessioniPrimary (citable) accession number: A4WX62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health