A4WVE5 (ISPE_RHOS5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Rsph17025_2471

Organism

Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) [Complete proteome] [HAMAP]

Taxonomic identifier

349102 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007883
Regions
	Nucleotide binding	89 – 99	11	ATP Potential
Sites
	Active site	9	1	By similarity
	Active site	128	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WVE5 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 14C8BB7F3CA1B80B	FASTA	278	28,901
	10 20 30 40 50 60 MTEAFARAKI NLTLHVTGQR PDGYHLLDSL VVFADVGDRV RTEPAEALSL AITGPQAVNL 70 80 90 100 110 120 PVSDDNLVLR AARAMGGQGA RITLEKHLPV ASGIGGGSAD AAATLKALAE LWQRPLPEAA 130 140 150 160 170 180 AVLRLGADVP VCLEGRAVRM AGVGEILEPL AGPLPPAWLV LANPGVSVPT PPVFKALARR 190 200 210 220 230 240 DNPPMPERLP DWGSADELAA FLAAMRNDLE APAIALAPEV AETRAALAAE PGCLIARMSG 250 260 270 SGATCFGLFA EERPARAAAE ALRAAHPGWW IEPARMAG

References

[1]

"Complete sequence of chromosome of Rhodobacter sphaeroides ATCC 17025." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17025 / ATH 2.4.3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000661 Genomic DNA. Translation: ABP71359.1.
RefSeq	YP_001168664.1. NC_009428.1.
3D structure databases
ProteinModelPortal	A4WVE5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WVE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5083814.
GenomeReviews	Gene locus Rsph17025_2471 in contig CP000661_GR.
KEGG	rsq:Rsph17025_2471.
PATRIC	23163273. VBIRhoSph94549_2552.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	VSTAEVY.
PhylomeDB	A4WVE5.
ProtClustDB	PRK14608.
Enzyme and pathway databases
BioCyc	RSPH349102:RSPH17025_2471-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_RHOS5

Accession

Primary (citable) accession number: A4WVE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program