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Protein

Isocitrate dehydrogenase [NADP]

Gene

Rsph17025_2367

Organism
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei109 – 1091SubstrateUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Sitei139 – 1391Critical for catalysisUniRule annotation
Sitei210 – 2101Critical for catalysisUniRule annotation
Metal bindingi250 – 2501Magnesium or manganeseUniRule annotation
Binding sitei258 – 2581NADPUniRule annotation
Metal bindingi273 – 2731Magnesium or manganeseUniRule annotation
Binding sitei326 – 3261NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPUniRule annotation
Nucleotide bindingi308 – 3136NADPUniRule annotation

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationImported

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciRSPH349102:GHE1-3694-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:Rsph17025_2367Imported
OrganismiRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)Imported
Taxonomic identifieri349102 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000000234 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi349102.Rsph17025_2367.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
KOiK00031.
OMAiGPKEWEV.
OrthoDBiEOG628F3X.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4WV42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIKVANPV VELDGDEMTR IIWDFIKQKL ILPYLDIDLH YYDLGIEERD
60 70 80 90 100
RTEDQITVDA ANAIKQYGVG VKCATITPDE ARVEEFGLKQ MWKSPNGTIR
110 120 130 140 150
NILGGVIFRQ PIICKNVPRL VPGWTKPIVV GRHAFGDQYR ATDFRFPGKG
160 170 180 190 200
KLTLKFVGED GTVIEREVFD APGAGVTMAM YNLDESILDF ARASMNYGLN
210 220 230 240 250
LGWPVYLSTK NTILKAYDGR FKDLFQKVYE EEFADRFKAA GITYEHRLID
260 270 280 290 300
DMVASALKWS GGYVWACKNY DGDVQSDTVA QGFGSLGLMT SVLMTPDGKT
310 320 330 340 350
VEAEAAHGTV TRHYRQHQAG KETSTNSIAS IFAWTGGLKH RAKLDDNAEL
360 370 380 390 400
MRFAETLERV TVQAVEDGFM TKDLALLVGP DQKWLTTMGY LEKVDEYLDR

ALGA
Length:404
Mass (Da):45,271
Last modified:May 29, 2007 - v1
Checksum:i57DCEDD585FC6DBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000661 Genomic DNA. Translation: ABP71256.1.
RefSeqiYP_001168561.1. NC_009428.1.

Genome annotation databases

EnsemblBacteriaiABP71256; ABP71256; Rsph17025_2367.
KEGGirsq:Rsph17025_2367.
PATRICi23163059. VBIRhoSph94549_2445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000661 Genomic DNA. Translation: ABP71256.1.
RefSeqiYP_001168561.1. NC_009428.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349102.Rsph17025_2367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP71256; ABP71256; Rsph17025_2367.
KEGGirsq:Rsph17025_2367.
PATRICi23163059. VBIRhoSph94549_2445.

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
KOiK00031.
OMAiGPKEWEV.
OrthoDBiEOG628F3X.

Enzyme and pathway databases

BioCyciRSPH349102:GHE1-3694-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17025 / ATH 2.4.3Imported.

Entry informationi

Entry nameiA4WV42_RHOS5
AccessioniPrimary (citable) accession number: A4WV42
Entry historyi
Integrated into UniProtKB/TrEMBL: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 29, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.