A4WTG2 (A4WTG2_RHOS5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2 HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2 HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme 2 HAMAP MF_00685
Glycogen branching enzyme 2 HAMAP MF_00685

Gene names

Name:	glgB2 HAMAP MF_00685
Ordered Locus Names:	Rsph17025_1783

Organism

Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) [Complete proteome] [HAMAP]

Taxonomic identifier

349102 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	409	1	Nucleophile By similarity HAMAP MF_00685
	Active site	462	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	A4WTG2 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: ECBE7002A839E99C	FASTA	728	81,317
	10 20 30 40 50 60 MNVTPKAEDL VPESDVAAIM RGTHGDPFGV LGMHGGGGTP LSVRVFAPQA AEVRVLDGAG 70 80 90 100 110 120 QPVATLDRIG AEGFFAGIVP GDEPFAYRLR FCSGPHEWEA EDPYRFPAVL GELDEYLMGE 130 140 150 160 170 180 GRHYQLYTRL GAHPVTLEGV EGVSFAVWAP SARRVSVVGA FNAWDGRRHP MRKRIGVGVW 190 200 210 220 230 240 ELFIPGLQVG DLYKYEILGP SGERLPLKSD PLSFAQEAPP ATASLVHGLP EPDWHDAGWM 250 260 270 280 290 300 QERASRQDRS APISIYEVHA GSWKLGLDYD ALAEELATYV REMGFTHVEF LPISEHPFTG 310 320 330 340 350 360 SWGYQPIGLF APTARFGPPE GFARLVDRLH REGIGVILDW VPAHFPSDAH GLANFDGTHL 370 380 390 400 410 420 YDHADPRQGF HRDWNTQIYN FGRQEVANFL QASALFWLDR YHVDALRVDA VASMLYLDYS 430 440 450 460 470 480 RNAGEWVPNR HGGRENLEAI DFLRAVNERV RIDHPGCITI AEESTAFPQV SRPVQEGGLG 490 500 510 520 530 540 FGFKWNMGWM HDTLGYFRRD PIHRRHHQND LTFGMVYAFS EDFVLPLSHD EVVHGKGSLI 550 560 570 580 590 600 GQMAGDRWQK FANLRAYFGF MWAHPGKKLL FMGGEFAQER EWNHDASLDW HLLDDPAHSG 610 620 630 640 650 660 MKRLVADLNR EYRRLPALHR MDCDPEGFEW IDAGDTENSV LSFLRKAPGE KPVLAVCNLT 670 680 690 700 710 720 PVVRSDYRIG VPQGGEWREI LNSDAAIYGG SDVGNPGSLQ AEEVSWHGRP FSVRLTLPPL ATILVTPA

References

[1]

"Complete sequence of chromosome of Rhodobacter sphaeroides ATCC 17025." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17025 / ATH 2.4.3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000661 Genomic DNA. Translation: ABP70676.1.
RefSeq	YP_001167981.1. NC_009428.1.
3D structure databases
ProteinModelPortal	A4WTG2.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WTG2.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5083689.
GenomeReviews	Gene locus Rsph17025_1783 in contig CP000661_GR.
KEGG	rsq:Rsph17025_1783.
PATRIC	23161846. VBIRhoSph94549_1845.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	RVYHQNG.
PhylomeDB	A4WTG2.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	RSPH349102:RSPH17025_1783-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4WTG2_RHOS5

Accession

Primary (citable) accession number: A4WTG2

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)