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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187SubstrateUniRule annotation1
Active sitei188Proton donor/acceptorUniRule annotation1
Active sitei318UniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Sitei331Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Rsph17025_0721Imported
OrganismiRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)Imported
Taxonomic identifieri349102 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000000234 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi349102.Rsph17025_0721.

Structurei

3D structure databases

ProteinModelPortaliA4WQG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 342Lyase_1InterPro annotationAdd BLAST331
Domaini408 – 461FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Substrate bindingUniRule annotation3
Regioni129 – 132Substrate binding (B site)UniRule annotation4
Regioni139 – 141Substrate bindingUniRule annotation3
Regioni324 – 326Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A4WQG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATRTETDS FGPLEVPADK YWGAQTQRSI QNFPIGWERQ PVAIIRALGV
60 70 80 90 100
VKKACALVNV DQGDMPKELG EAIAAAATEV IEGRFDDNFP LVVWQTGSGT
110 120 130 140 150
QSNMNANEVI SNRAIEMLGG VMGSKKPVHP NDHVNMGQSS NDTFPTAMHV
160 170 180 190 200
AIGMMARDVL LPGLEKLHAA LLAKSEEFKD IIKIGRTHTQ DATPLTLGQE
210 220 230 240 250
FSGYAKQVEK GIARVKACLP DIYELAQGGT AVGTGLNTRQ GWDSRVAGKI
260 270 280 290 300
AEITGLPFVT AENKFEALAA HDAMVMFSGA LKTVAASLFK IANDMRLLGS
310 320 330 340 350
GPRSGLGELI LPENEPGSSI MPGKVNPTQA EALTMVCAHV MGNDAAVGFA
360 370 380 390 400
GSQGHFELNV YNPMMSYNVL QSMQLLGDAA GSFTDNMVVG TQANTARIDK
410 420 430 440 450
LMKESLMLVT ALAPTIGYDN ATKVAKTAHK NGTTLREEAI ALGFVDAETF
460
DRIVRPEDMI GPKG
Length:464
Mass (Da):49,489
Last modified:May 29, 2007 - v1
Checksum:i1208DFDEFFEA5032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000661 Genomic DNA. Translation: ABP69627.1.
RefSeqiWP_011907774.1. NC_009428.1.

Genome annotation databases

EnsemblBacteriaiABP69627; ABP69627; Rsph17025_0721.
KEGGirsq:Rsph17025_0721.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA4WQG3_RHOS5
AccessioniPrimary (citable) accession number: A4WQG3
Entry historyiIntegrated into UniProtKB/TrEMBL: May 29, 2007
Last sequence update: May 29, 2007
Last modified: June 7, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported