Reviewed, UniProtKB/Swiss-Prot A4WPH4 (SYE1_RHOS5)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Rsph17025_0382

Organism

Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) [Complete proteome] [HAMAP]

Taxonomic identifier

349102 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367745
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WPH4-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 67190476E1D4564B	FASTA	441	49,046
	10 20 30 40 50 60 MTTVTRFAPS PTGFIHVGNL RTALMNWAIA RKSGGTFILR LDDTDRERSK QEYSDAIMQD 70 80 90 100 110 120 LEWLGLTWDR LERQSDRLDR YAEAAGDLRR AGRFYECFES PTELDLKRKK LLNMGKPPVY 130 140 150 160 170 180 DRAALKLSDE DRARLREERG GYWRFLLDQE RIEWTDGILG PISIDAASVS DPVLIRADGQ 190 200 210 220 230 240 VLYTFASSVD DIDMGVTFIV RGADHVTNTA TQIQIMQAMG GTPPSFAHHS LLTGAQGEAL 250 260 270 280 290 300 SKRLGTLSLR DLRARGVEPM ALLSLMARLG SSQPVELFRT HEELLAGFDV GTFGAAPTKF 310 320 330 340 350 360 DAEDLFPLTR HYVQGLPFEA VAERIRSLGV PDALAEPFWR VAKDNIAVLE DLGGWWTLFS 370 380 390 400 410 420 EGAEPQIDPE DADFIRQAMA LLPEPPYGPE TWGQWTAAVK EATGRKGKGL FMPLRKALTG 430 440 QAHGPEMADV MPLLQTVRAK G

References

[1]

"Complete sequence of chromosome of Rhodobacter sphaeroides ATCC 17025." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000661 Genomic DNA. Translation: ABP69288.1.
RefSeq	YP_001166593.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4WPH4.
Genome annotation databases
GeneID	5082218.
GenomeReviews	Gene locus Rsph17025_0382 in contig CP000661_GR.
KEGG	rsq:Rsph17025_0382.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LRLDDTD.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_RHOS5

Accession

Primary (citable) accession number: A4WPH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)