A4WNU5 (A4WNU5_RHOS5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Peptide deformylase 1 HAMAP MF_00163
Short name=PDF 1 HAMAP MF_00163
EC=3.5.1.88 HAMAP MF_00163

Alternative name(s):
Polypeptide deformylase 1 HAMAP MF_00163

Gene names

Name:	def1 HAMAP MF_00163
Ordered Locus Names:	Rsph17025_0148

Organism

Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) [Complete proteome] [HAMAP]

Taxonomic identifier

349102 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163 SAAS SAAS000181
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family. HAMAP MF_00163 RuleBase RU003335

Ontologies

Keywords
Biological process	Protein biosynthesis SAAS SAAS000181 HAMAP MF_00163
Ligand	Iron HAMAP MF_00163 Metal-binding HAMAP MF_00163 SAAS SAAS000181
Molecular function	Hydrolase HAMAP MF_00163 SAAS SAAS000181 EMBL ABP69059.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	137	1	By similarity HAMAP MF_00163
	Metal binding	94	1	Iron By similarity HAMAP MF_00163
	Metal binding	136	1	Iron By similarity HAMAP MF_00163
	Metal binding	140	1	Iron By similarity HAMAP MF_00163

Sequences

	Sequence		Length	Mass (Da)
	A4WNU5 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 0B9FC8B6EABF1F20	FASTA	177	20,183
	10 20 30 40 50 60 MIRSILIHPD PRLKKICDPV AEITDDLRRL ADDMLATMYD APGIGLAAPQ VGVMRRLVVM 70 80 90 100 110 120 DCNKQPEAPR RPIAMINPQV VWASEDLSTY EEGCLSLPNV FAEVERPAEV KVRWTGIDGR 130 140 150 160 170 EEEEQFSGLW ATCVQHEIDH LDGKLFIDYL RPLKRQMITR KMEKFRRAQA SGLVQKA

References

[1]

"Complete sequence of chromosome of Rhodobacter sphaeroides ATCC 17025." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Richardson P., Mackenzie C., Choudhary M., Donohue T.J., Kaplan S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17025 / ATH 2.4.3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000661 Genomic DNA. Translation: ABP69059.1.
RefSeq	YP_001166364.1. NC_009428.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4WNU5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5082556.
GenomeReviews	Gene locus Rsph17025_0148 in contig CP000661_GR.
KEGG	rsq:Rsph17025_0148.
PATRIC	23158402. VBIRhoSph94549_0146.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0242.
HOGENOM	HBG665227.
OMA	GVLFVDY.
PhylomeDB	A4WNU5.
ProtClustDB	CLSK934098.
Enzyme and pathway databases
BioCyc	RSPH349102:RSPH17025_0148-MONOMER.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4WNU5_RHOS5

Accession

Primary (citable) accession number: A4WNU5

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)