A4WNC5 (PIMT_PYRAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	Pars_2349

Organism

Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]

Taxonomic identifier

340102 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum › Pyrobaculum arsenaticum

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP-Rule MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP-Rule MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Protein-L-isoaspartate O-methyltransferase HAMAP-Rule MF_00090		PRO_1000093267
Sites
	Active site	56	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WNC5 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 5F8CC2883F2F557C	FASTA	205	22,689
	10 20 30 40 50 60 MAKKLVDDLE REGVLKSERV KKALLSVPRE EFVMPEYRMM AYEDRPLPLF ADATISAPHM 70 80 90 100 110 120 VAMMCELIEP RPGMSILEVG TGSGYHAAVC AEAIERRGKV YTVEIVKGLA IYAAQNLERL 130 140 150 160 170 180 GYWGVVEVFH SDGKRGLEKF APYDAIIVTA AAASIPSALV NQLKDGGIMV IPVEEGFGQV 190 200 LYKVVRRGEK TEKKAVTYVL FVPLR

References

[1]

"Complete sequence of Pyrobaculum arsenaticum DSM 13514." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13514 / JCM 11321.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000660 Genomic DNA. Translation: ABP51892.1.
RefSeq	YP_001154544.1. NC_009376.1.
3D structure databases
ProteinModelPortal	A4WNC5.
ModBase	Search...
Protein-protein interaction databases
STRING	340102.Pars_2349.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABP51892; ABP51892; Pars_2349.
GeneID	5056122.
KEGG	pas:Pars_2349.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
KO	K00573.
OMA	HYIKYFP.
ProtClustDB	PRK13944.
Enzyme and pathway databases
BioCyc	PARS340102:GHGQ-2385-MONOMER.
Family and domain databases
HAMAP	MF_00090. PIMT.
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_PYRAR

Accession

Primary (citable) accession number: A4WNC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 29, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program