A4WN46 (ILVD_PYRAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Pars_2269

Organism

Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]

Taxonomic identifier

340102 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000001040
Sites
	Metal binding	124	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	196	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A4WN46 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: C3FAA81F32B817B2	FASTA	564	59,929
	10 20 30 40 50 60 MVKLRIRSSQ WYDGVDNAPH RPYLRAVGLT EADFAKPLVG VLVSWSELGP CNFHNLELVR 70 80 90 100 110 120 YVKEGVKEAG GVGLAAPTIV VNDGINMGTP GMRYSLISRD LIADTIEAQF NSHGVDAWVG 130 140 150 160 170 180 IGGCDKTQPG IMMAMVRLDL PAVYLYGGSA EAGWLGEREL TIEDAFESVG AYLAGKITLD 190 200 210 220 230 240 ELKRVEELSF PTYGTCQGMF TANTMATLGE ALGLSLLGSA SPPATSARRR KYAVESGRAV 250 260 270 280 290 300 LKAAELGVTP RKVVTYDALY NAAVTLFATA GSTNAILHLL AIAHEANVKF TLDDFDEISR 310 320 330 340 350 360 RVPVIAALRP AGPYAMQDLD RIGGVPRVLK KLYKAGLLRP EALTVEGEPI GKLLERWEPP 370 380 390 400 410 420 AVPEAGILYD VEKPYKPYSG IRILRGNLAP SGAVMKIGAA DKLRFEGRAK VYDSEAEAFK 430 440 450 460 470 480 AVAAGEIKPG DVVIIRYEGP KGAPGMPEML KVTAAIVGAG LGDAVALVTD GRFSGATRGI 490 500 510 520 530 540 MVGHVAPEAA VGGPIALVQN GDRVIIDGEA GLIKLEVSEE ELEKRRKAWA PPPPKYKGGL 550 560 LAKYAALVQQ ADKGAVTSPS AWGT

References

[1]

"Complete sequence of Pyrobaculum arsenaticum DSM 13514." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13514 / JCM 11321.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000660 Genomic DNA. Translation: ABP51813.1.
RefSeq	YP_001154465.1. NC_009376.1.
3D structure databases
ProteinModelPortal	A4WN46.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WN46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5055399.
GenomeReviews	Gene locus Pars_2269 in contig CP000660_GR.
KEGG	pas:Pars_2269.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04340.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	A4WN46.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	PARS340102:PARS_2269-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_PYRAR

Accession

Primary (citable) accession number: A4WN46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program