A4WN26 (A4WN26_PYRAR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase RuleBase RU000515
EC=4.2.1.24 RuleBase RU000515

Gene names

Ordered Locus Names:

Pars_2247

Organism

Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]

Taxonomic identifier

340102 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O. RuleBase RU000515
Subunit structure	Homooctamer By similarity. PIRSR PIRSR001415-3 RuleBase RU000515
Sequence similarities	Belongs to the ALADH family. RuleBase RU004161

Ontologies

Keywords
Biological process	Porphyrin biosynthesis RuleBase RU000515
Ligand	Magnesium PIRSR PIRSR001415-5 Metal-binding PIRSR PIRSR001415-5 Zinc PIRSR PIRSR001415-3
Molecular function	Lyase RuleBase RU000515 EMBL ABP51793.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	204	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Active site	257	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Metal binding	125	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	127	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	135	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	242	1	Magnesium By similarity PIRSR PIRSR001415-5
	Binding site	214	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	226	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	284	1	Substrate 2 By similarity PIRSR PIRSR001415-2
	Binding site	323	1	Substrate 2 By similarity PIRSR PIRSR001415-2

Sequences

	Sequence		Length	Mass (Da)
	A4WN26 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 9E155435B05E66F1	FASTA	338	37,577
	10 20 30 40 50 60 MHVRFPQHRP RRLRASKLIR DAVAETSLDP SDFIYPIFVK PSGEKEQIPS MPGQYRWPVG 70 80 90 100 110 120 DELTRHVEEA LALGVNKVIL FGVVPDELKD SAGSPGYDPH GVVPNAIRLL KQTFGDKLLV 130 140 150 160 170 180 FADVCLCEYT DHGHCGIVRE RRGRWYVDND ETIKLYAKEA VTYADAGADF VAPSGMMDGQ 190 200 210 220 230 240 VAEIRKALDA HGFHDVGIMA YSAKYASAFY GPFRVAAASA PKFGDRRTYQ MDPRNAYEAV 250 260 270 280 290 300 KEVMLDLEEG ADIVMVKPAL AYLDVIRLVK THYPWAPLAA YNVSGEYSMV KAAASLGYVD 310 320 330 ERIVTLEILT AIKRAGAQLI LTYHALEAAR WLKEGVPF

References

[1]

"Complete sequence of Pyrobaculum arsenaticum DSM 13514." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13514 / JCM 11321.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000660 Genomic DNA. Translation: ABP51793.1.
RefSeq	YP_001154445.1. NC_009376.1.
3D structure databases
ProteinModelPortal	A4WN26.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WN26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5055067.
GenomeReviews	Gene locus Pars_2247 in contig CP000660_GR.
KEGG	pas:Pars_2247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05029.
HOGENOM	HBG285270.
OMA	ADLCFCE.
PhylomeDB	A4WN26.
ProtClustDB	PRK09283.
Enzyme and pathway databases
BioCyc	PARS340102:PARS_2247-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4WN26_PYRAR

Accession

Primary (citable) accession number: A4WN26

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)