A4WMV7 (PURL_PYRAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Pars_2178

Organism

Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]

Taxonomic identifier

340102 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050337
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A4WMV7 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 4098D2893C747B03	FASTA	697	74,613
	10 20 30 40 50 60 MALSREELEV IERGLGRPPS EVELALFRSH WSEHCSYKST RMWLRRLPSR APWVVRGPGT 70 80 90 100 110 120 DAPLVEIAEG LFVTFKIESH NHPSAVDPYN GAATGVGGII RDILTVGARP VALLVNLHFG 130 140 150 160 170 180 PLDHPHARWI ASNVVRGISD YGNRVGVPVV GGETWFDEDF TYTPIVLATC VGVVEKGKVP 190 200 210 220 230 240 PGGVETGDLI VVAGLGADKS GLGGSAFASK TLREESEEDL GAVQVADPLM GKKLIDLVQE 250 260 270 280 290 300 AVGCVKFIKD LGGGGLATAL AELAEWFGLG VEAELDKIHM SDREMGPAEV LSSETQERLV 310 320 330 340 350 360 FVVSPQGLEC LKSLLEKYEV PYSVVGRFVK SGRVRLLWRG EVVGDVPVWL AAKAPEMVWP 370 380 390 400 410 420 RESYEPPPLP EIPEPPIDKA VELVLSSPNV AVKEAIYRRF DFDVGVRTAV KPGEGDAAVL 430 440 450 460 470 480 KLYERGNLGI VVKGDANPRY AYLDPRLGAA NAFVKAYRNV AVAGGVPKAA VDSINVGSPA 490 500 510 520 530 540 RPAVYWQFVE AVEGLKEAAE ALGVPIVGGK VSLYNEYGDR PIKPTVAVVV LGVIDDVSTA 550 560 570 580 590 600 LRALWRDGDG VYLWGFTRGE VGGSEYMYRV HRLVAGRPPS VDYAAEKRIV EEVQRWRGRL 610 620 630 640 650 660 TGAKDVGVGG LAAALAKMAV ASGVGADVDV CKAPTDMGRL DYLLFSESNG RFVAAGEEGP 670 680 690 GVKIGVAGGD RLVLRCGSTQ LFSRSVDGLK ELMSLGL

References

[1]

"Complete sequence of Pyrobaculum arsenaticum DSM 13514." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13514 / JCM 11321.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000660 Genomic DNA. Translation: ABP51724.1.
RefSeq	YP_001154376.1. NC_009376.1.
3D structure databases
ProteinModelPortal	A4WMV7.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WMV7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5055830.
GenomeReviews	Gene locus Pars_2178 in contig CP000660_GR.
KEGG	pas:Pars_2178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	PARS340102:PARS_2178-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRAR

Accession

Primary (citable) accession number: A4WMV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program