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A4WL99 (SYW_PYRAR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:Pars_1612
OrganismPyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]
Taxonomic identifier340102 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum ›

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_1000018996

Regions

Motif81 – 899"HIGH" region HAMAP-Rule MF_00140
Motif258 – 2625"KMSKS" region HAMAP-Rule MF_00140

Sequences

Sequence LengthMass (Da)Tools
A4WL99 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 6720CBFF77A8635C

FASTA37442,636
        10         20         30         40         50         60 
MGEDFVVTPW EVRGKVDYEK LLRQFGAKPL TAEEVALLEK YAGDVHPLIK RGFFYAHRDF 

        70         80         90        100        110        120 
DFILKWHGEG RPWALYTGRG PSGPVHIGHM VPWILLKWFS DKFGVEVYFQ MTDDEKFFDD 

       130        140        150        160        170        180 
PEMKLEEATG WAYENALDVI ALGFGPDKLH LIVDTKDIAP LYPIAVRVAK KLTWNTVKAT 

       190        200        210        220        230        240 
FGFTDSSNIG LIFYPSLQIA VAFLPTELKK EPTPVLIPCA IDQDPYFRLA RDIADSLSYP 

       250        260        270        280        290        300 
KPTTLYSKFI MALTGESKMS ASNPDSAIYT MDDDKTVKHK ILNAFTGGRP TAEEQRKYGG 

       310        320        330        340        350        360 
NPDICPVFHY HMLFDPDDAS VEKIRQDCKS GALLCGECKL KLHEKISKFL KEHRERREKA 

       370 
RGKVDEYRLS VKLK

« Hide

References

[1]"Complete sequence of Pyrobaculum arsenaticum DSM 13514."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13514 / JCM 11321.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000660 Genomic DNA. Translation: ABP51166.1.
RefSeqYP_001153818.1. NC_009376.1.

3D structure databases

ProteinModelPortalA4WL99.
ModBaseSearch...

Protein-protein interaction databases

STRING340102.Pars_1612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP51166; ABP51166; Pars_1612.
GeneID5056275.
KEGGpas:Pars_1612.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000224742.
KOK01867.
OMAKTGGRET.
ProtClustDBPRK12285.

Enzyme and pathway databases

BioCycPARS340102:GHGQ-1650-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_A. Trp_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_PYRAR
AccessionPrimary (citable) accession number: A4WL99
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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