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A4WK38

- LIPA_PYRAR

UniProt

A4WK38 - LIPA_PYRAR

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Protein
Probable lipoyl synthase
Gene
lipA, Pars_1184
Organism
Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi39 – 391Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi45 – 451Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi60 – 601Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi64 – 641Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPARS340102:GHGQ-1198-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Pars_1184
OrganismiPyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Taxonomic identifieri340102 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
ProteomesiUP000001567: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Probable lipoyl synthaseUniRule annotation
PRO_0000325323Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi340102.Pars_1184.

Structurei

3D structure databases

ProteinModelPortaliA4WK38.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A4WK38-1 [UniParc]FASTAAdd to Basket

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MSALPAWLRV EARSYSEMAK VRSVLSRLGV YTVCEGARCP NVFRCWGEGT    50
ATFMILGEVC TRACRFCSVR TGNPRGYVDL DEPRRVAEAV RRLGLRYVVV 100
TSVDRDDLPD GGAFQYASAI REIRRLAPGA LVEVLTPDFR GSREAVETVA 150
EAGPDVFAHN VETVRRLTPL VRDRRASYET SLRVLKTAKE VGCRLTKSGI 200
MLGLGESFDE VVEVLDDLRK AEVDIVTIGQ YVRPTKSARH LPVARWVPLE 250
EFQKLGEVAL SMGFKAVASA PLVRSSYRAE DLYEMALGLR PRAVLV 296
Length:296
Mass (Da):32,853
Last modified:May 29, 2007 - v1
Checksum:i917D6702E0B7AC22
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000660 Genomic DNA. Translation: ABP50755.1.
RefSeqiYP_001153407.1. NC_009376.1.

Genome annotation databases

EnsemblBacteriaiABP50755; ABP50755; Pars_1184.
GeneIDi5055526.
KEGGipas:Pars_1184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000660 Genomic DNA. Translation: ABP50755.1 .
RefSeqi YP_001153407.1. NC_009376.1.

3D structure databases

ProteinModelPortali A4WK38.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 340102.Pars_1184.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP50755 ; ABP50755 ; Pars_1184 .
GeneIDi 5055526.
KEGGi pas:Pars_1184.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PARS340102:GHGQ-1198-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13514 / JCM 11321.

Entry informationi

Entry nameiLIPA_PYRAR
AccessioniPrimary (citable) accession number: A4WK38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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