Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A4WK38

- LIPA_PYRAR

UniProt

A4WK38 - LIPA_PYRAR

Protein

Probable lipoyl synthase

Gene

lipA

Organism
Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi34 – 341Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi39 – 391Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi45 – 451Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi64 – 641Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPARS340102:GHGQ-1198-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Pars_1184
    OrganismiPyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
    Taxonomic identifieri340102 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
    ProteomesiUP000001567: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296Probable lipoyl synthasePRO_0000325323Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi340102.Pars_1184.

    Structurei

    3D structure databases

    ProteinModelPortaliA4WK38.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235998.
    KOiK03644.
    OMAiHPHIPTK.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4WK38-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALPAWLRV EARSYSEMAK VRSVLSRLGV YTVCEGARCP NVFRCWGEGT    50
    ATFMILGEVC TRACRFCSVR TGNPRGYVDL DEPRRVAEAV RRLGLRYVVV 100
    TSVDRDDLPD GGAFQYASAI REIRRLAPGA LVEVLTPDFR GSREAVETVA 150
    EAGPDVFAHN VETVRRLTPL VRDRRASYET SLRVLKTAKE VGCRLTKSGI 200
    MLGLGESFDE VVEVLDDLRK AEVDIVTIGQ YVRPTKSARH LPVARWVPLE 250
    EFQKLGEVAL SMGFKAVASA PLVRSSYRAE DLYEMALGLR PRAVLV 296
    Length:296
    Mass (Da):32,853
    Last modified:May 29, 2007 - v1
    Checksum:i917D6702E0B7AC22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000660 Genomic DNA. Translation: ABP50755.1.
    RefSeqiYP_001153407.1. NC_009376.1.

    Genome annotation databases

    EnsemblBacteriaiABP50755; ABP50755; Pars_1184.
    GeneIDi5055526.
    KEGGipas:Pars_1184.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000660 Genomic DNA. Translation: ABP50755.1 .
    RefSeqi YP_001153407.1. NC_009376.1.

    3D structure databases

    ProteinModelPortali A4WK38.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 340102.Pars_1184.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP50755 ; ABP50755 ; Pars_1184 .
    GeneIDi 5055526.
    KEGGi pas:Pars_1184.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235998.
    KOi K03644.
    OMAi HPHIPTK.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci PARS340102:GHGQ-1198-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13514 / JCM 11321.

    Entry informationi

    Entry nameiLIPA_PYRAR
    AccessioniPrimary (citable) accession number: A4WK38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3