A4WJ34 (RIFK_PYRAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Pars_0815

Organism

Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]

Taxonomic identifier

340102 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Riboflavin kinase HAMAP MF_01285		PRO_0000322100
Regions
	Nucleotide binding	91 – 96	6	CDP By similarity
	Nucleotide binding	190 – 193	4	CDP By similarity
	Region	1 – 81	81	H-T-H motif-like HAMAP MF_01285
	Region	82 – 210	129	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	120	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Binding site	177	1	FMN By similarity
	Binding site	185	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WJ34 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: DC555FBC87E374DC	FASTA	210	23,188
	10 20 30 40 50 60 MECRERRLAA DLIALSSVEG LPVSEAAKRL CMTRQGLYKL LKQLRAEGYV AEGSVVKLTP 70 80 90 100 110 120 KGRDFLSGIL RDLLRYFNIA SIRLVGRVVS GLGEGAFYIS LEGYKRAIEE RLGFTPFPGT 130 140 150 160 170 180 LNIKLDPQYM PYRRYLDGLP GVVIPGFSNG LRTYGAVKAF RARVNGVEGA VVMPERTHHP 190 200 210 TDVIEVVAPV KLRDALGLRD GDVVEVEVLL

References

[1]

"Complete sequence of Pyrobaculum arsenaticum DSM 13514." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13514 / JCM 11321.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000660 Genomic DNA. Translation: ABP50401.1.
RefSeq	YP_001153053.1. NC_009376.1.
3D structure databases
ProteinModelPortal	A4WJ34.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WJ34.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5055447.
GenomeReviews	Gene locus Pars_0815 in contig CP000660_GR.
KEGG	pas:Pars_0815.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	MPERTHH.
ProtClustDB	CLSK631895.
Enzyme and pathway databases
BioCyc	PARS340102:PARS_0815-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRAR

Accession

Primary (citable) accession number: A4WJ34

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program