Reviewed, UniProtKB/Swiss-Prot A4WIW2 (G3P_PYRAR)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Pars_0743

Organism

Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]

Taxonomic identifier

340102 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000300975
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	139 – 141	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	140	1	Nucleophile By similarity
	Binding site	110	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	302	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WIW2-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 02B316F2B8F47E36	FASTA	344	37,492
	10 20 30 40 50 60 MIRVGIVGFG TIGKRIADAV AAQGDMHVSG VLKVTPDYEV LVAAAKGFKV YTLPDRVEKF 70 80 90 100 110 120 KKAGIEPAGT IEDLIKASDV IIDASPEDVG AENKEKYYSK FDKPVIFQGG EEAEVAEVSF 130 140 150 160 170 180 NALANYDEAR GRRYVRVVSC NTTGITRVLT SLMLNGVGIK KARIFIARRG ADPKEYKKGP 190 200 210 220 230 240 INDVVPNPAT VPSHHGPDVK TVLKNVDIVT MAVAVPVTIM HMHMAFLELD GAYPRDAVLE 250 260 270 280 290 300 ALAKTPRIFL ADVGAGFQSL AQIIEYARDL GRPRGDFPEV AIFRDSVTVN GNELYLMYGV 310 320 330 340 HQESIVVPEN VDAVRAVLGA MPKWESIKKT DTTLKLFTEG KRYG

References

[1]

"Complete sequence of Pyrobaculum arsenaticum DSM 13514." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000660 Genomic DNA. Translation: ABP50329.1.
RefSeq	YP_001152981.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5054487.
GenomeReviews	Gene locus Pars_0743 in contig CP000660_GR.
KEGG	pas:Pars_0743.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4WIW2. AIFQGGE.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_PYRAR

Accession

Primary (citable) accession number: A4WIW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)