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A4WHK6 (SYL_PYRAR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pars_0260
OrganismPyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]
Taxonomic identifier340102 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length945 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 945945Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009403

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif638 – 6425"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4WHK6 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: FBF96F309DE85956

FASTA945107,394
        10         20         30         40         50         60 
MSELARFFIE LGEKWQKRWA EARVYEPAPT LGVPKFFITA AYPYPNGAIH IGHGRTYLIA 

        70         80         90        100        110        120 
DVLARFHRHL GRAVLFPMAF HYTGTPILTI AEAIAAGDET VVEEYMAIYG VPEEEMRKMG 

       130        140        150        160        170        180 
DPLYLARYFH EQSKRAMQKF GLSIDWTREF TTIDPEYQRF IQWQFEKLRK KGLIVRGRHP 

       190        200        210        220        230        240 
VGWCPRHSMP VGAHDTKDDK EPDIGEWTLI YFADRDGLIF PAATLRPETV LGVTNMWINP 

       250        260        270        280        290        300 
EGEYVVAEYD GRKMVLSRDA AYRLSFQGSV KVLREAKGRE FVGREVQNPV TGEWVPIYGA 

       310        320        330        340        350        360 
KFVDPKVGTG VVMSVPAHAP YDYAALRDIG AIRLIPLIKV EGYGEYPAKD VVERMEIKSQ 

       370        380        390        400        410        420 
TDPALEEATK EVYSAEYARG VMREDVVELV GRHLPEPARS MVMAVFKMYF AGRPVREARE 

       430        440        450        460        470        480 
FISKWLAEAG LGGVMYDIMN KPVYCRCGTE IVVKVLEDQW FINYGEPRWK ELAKKLVEEM 

       490        500        510        520        530        540 
TIVPPEAKAQ FFATIDWLDK RACARTRGLG TPLPWSHGWV IESLSDSTIY MAYYAVIKGI 

       550        560        570        580        590        600 
RRHNLRPEQL TEEFWDYVFL GVGTPEEVSA KTGIPAEALR AIREEFEFWY PLDSRNSGKD 

       610        620        630        640        650        660 
LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD KAVEMYGPDP 

       670        680        690        700        710        720 
LRATLAISAE VEQDLDFRHA EAVRNAQQLM SIYTLAQRLA QSAEDREPTW LDRWLLSEVA 

       730        740        750        760        770        780 
LALERVRDAY EKVRVRQAAV ELLYNIKNIF DSYMTAVERP SRLAVEVAKA WAVALEPIAP 

       790        800        810        820        830        840 
HLAEEVWSLL GGEGFVTSAK WPQLKPDPAA LLARRYVDMV VEDVKKIPAY GEGVRRVVLY 

       850        860        870        880        890        900 
INPNFTWVKA ALNNDVKSAI AAGTPPQLAK RLVELVRTLG DEVRSLIAAV ENFDEREALL 

       910        920        930        940 
SYKNYVEKAL GAPVEVYTAE DPAAPDLGGK KKAALPLKPG IFIER 

« Hide

References

[1]"Complete sequence of Pyrobaculum arsenaticum DSM 13514."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13514 / JCM 11321.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000660 Genomic DNA. Translation: ABP49873.1.
RefSeqYP_001152525.1. NC_009376.1.

3D structure databases

ProteinModelPortalA4WHK6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340102.Pars_0260.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP49873; ABP49873; Pars_0260.
GeneID5054166.
KEGGpas:Pars_0260.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIDKWLLH.

Enzyme and pathway databases

BioCycPARS340102:GHGQ-262-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PYRAR
AccessionPrimary (citable) accession number: A4WHK6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries