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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei641 – 6411ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPARS340102:GHGQ-262-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Pars_0260
OrganismiPyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Taxonomic identifieri340102 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
ProteomesiUP000001567 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Leucine--tRNA ligasePRO_1000009403Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA4WHK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi638 – 6425"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiYEGVMLV.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4WHK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELARFFIE LGEKWQKRWA EARVYEPAPT LGVPKFFITA AYPYPNGAIH
60 70 80 90 100
IGHGRTYLIA DVLARFHRHL GRAVLFPMAF HYTGTPILTI AEAIAAGDET
110 120 130 140 150
VVEEYMAIYG VPEEEMRKMG DPLYLARYFH EQSKRAMQKF GLSIDWTREF
160 170 180 190 200
TTIDPEYQRF IQWQFEKLRK KGLIVRGRHP VGWCPRHSMP VGAHDTKDDK
210 220 230 240 250
EPDIGEWTLI YFADRDGLIF PAATLRPETV LGVTNMWINP EGEYVVAEYD
260 270 280 290 300
GRKMVLSRDA AYRLSFQGSV KVLREAKGRE FVGREVQNPV TGEWVPIYGA
310 320 330 340 350
KFVDPKVGTG VVMSVPAHAP YDYAALRDIG AIRLIPLIKV EGYGEYPAKD
360 370 380 390 400
VVERMEIKSQ TDPALEEATK EVYSAEYARG VMREDVVELV GRHLPEPARS
410 420 430 440 450
MVMAVFKMYF AGRPVREARE FISKWLAEAG LGGVMYDIMN KPVYCRCGTE
460 470 480 490 500
IVVKVLEDQW FINYGEPRWK ELAKKLVEEM TIVPPEAKAQ FFATIDWLDK
510 520 530 540 550
RACARTRGLG TPLPWSHGWV IESLSDSTIY MAYYAVIKGI RRHNLRPEQL
560 570 580 590 600
TEEFWDYVFL GVGTPEEVSA KTGIPAEALR AIREEFEFWY PLDSRNSGKD
610 620 630 640 650
LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD
660 670 680 690 700
KAVEMYGPDP LRATLAISAE VEQDLDFRHA EAVRNAQQLM SIYTLAQRLA
710 720 730 740 750
QSAEDREPTW LDRWLLSEVA LALERVRDAY EKVRVRQAAV ELLYNIKNIF
760 770 780 790 800
DSYMTAVERP SRLAVEVAKA WAVALEPIAP HLAEEVWSLL GGEGFVTSAK
810 820 830 840 850
WPQLKPDPAA LLARRYVDMV VEDVKKIPAY GEGVRRVVLY INPNFTWVKA
860 870 880 890 900
ALNNDVKSAI AAGTPPQLAK RLVELVRTLG DEVRSLIAAV ENFDEREALL
910 920 930 940
SYKNYVEKAL GAPVEVYTAE DPAAPDLGGK KKAALPLKPG IFIER
Length:945
Mass (Da):107,394
Last modified:May 29, 2007 - v1
Checksum:iFBF96F309DE85956
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000660 Genomic DNA. Translation: ABP49873.1.
RefSeqiWP_011899781.1. NC_009376.1.
YP_001152525.1. NC_009376.1.

Genome annotation databases

EnsemblBacteriaiABP49873; ABP49873; Pars_0260.
GeneIDi5054166.
KEGGipas:Pars_0260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000660 Genomic DNA. Translation: ABP49873.1.
RefSeqiWP_011899781.1. NC_009376.1.
YP_001152525.1. NC_009376.1.

3D structure databases

ProteinModelPortaliA4WHK6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP49873; ABP49873; Pars_0260.
GeneIDi5054166.
KEGGipas:Pars_0260.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiYEGVMLV.

Enzyme and pathway databases

BioCyciPARS340102:GHGQ-262-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13514 / JCM 11321.

Entry informationi

Entry nameiSYL_PYRAR
AccessioniPrimary (citable) accession number: A4WHK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: June 24, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.