Reviewed, UniProtKB/Swiss-Prot A4WH24 (DNLI_PYRAR)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Pars_0076

Organism

Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) [Complete proteome] [HAMAP]

Taxonomic identifier

340102 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	DNA ligase		PRO_1000049875
Sites
	Active site	251	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WH24-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: D65376C5A129E101	FASTA	584	64,746
	10 20 30 40 50 60 MQFGELVKAL AAVESTTQRS VMVKLLASLF KKALPEEIDK VIYLILGDLR PPWEGLELGV 70 80 90 100 110 120 GEKLCLRALA KATGTSQAEL ESMYKKTGDI GEAARRALAS SKRPGLLAFG SQKPLEVSEV 130 140 150 160 170 180 YDALIKVARA TGEGAQDMKI ALLSSLFARS SPEEGKYIAR FVVGKLRLGV ADMTIIEALA 190 200 210 220 230 240 DAYGVRKEDL ERAYHVYPDL GHLAKLVASG KPLDEVKVTP GVPVLPMLAQ RLSSSSEILA 250 260 270 280 290 300 KLGGAAICEY KYDGERAQIH ISQSGVRIFS RRLEDITHAY PDVVKAVREA VSAREAILEG 310 320 330 340 350 360 EIVAVDPDTG EMLPFQELMH RKRKHEVAAA MEMYPTVLYL FDIVYVDGED LTNEPLIYRR 370 380 390 400 410 420 VKLSEIVHES DKVQIAKWQM FDDPDTVDVF FHEAVSVGTE GLICKSPTSE YEMGARGWNW 430 440 450 460 470 480 IKYKRDYKSE MIDTVDLVVV GAFYGRGKRA GLYGAFLLAA YDPSSDMFYT VCKVGSGFTD 490 500 510 520 530 540 ADLKKMYEIL QPLKIPHRHP RVSSKMEADV WFVPQVVIEV IGAEITLSPL HTCCLGAVRP 550 560 570 580 GVGLAVRFPR FTGRYRTDKG PEQATTVAEM LELYKRQKKV AQPE

References

[1]

"Complete sequence of Pyrobaculum arsenaticum DSM 13514." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000660 Genomic DNA. Translation: ABP49691.1.
RefSeq	YP_001152343.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5054375.
GenomeReviews	Gene locus Pars_0076 in contig CP000660_GR.
KEGG	pas:Pars_0076.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. [Graphical view]
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_PYRAR

Accession

Primary (citable) accession number: A4WH24

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)