A4WGE3 (ASNA_ENT38) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	Ent638_4120

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000061105

Sequences

	Sequence		Length	Mass (Da)
	A4WGE3 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 0D8079D1F820C70D	FASTA	330	36,689
	10 20 30 40 50 60 MKTAYIVKQR QISFVKSHFS RQLEEKLGLI EVQAPILSRV GDGTQDNLSG CEKAVQVKVK 70 80 90 100 110 120 TLPDAQFEVV HSLAKWKRQT LGQHDFSAGE GLYTHMKALR PDEDRLTAIH SVYVDQWDWE 130 140 150 160 170 180 RVMGDGERHV GTLKSTVEAI YAGIKATETA VSKEFGLTPF LPDTIHFIHS QDLLSRFPDL 190 200 210 220 230 240 DAKGRERAIA KELGAVFLIG IGGKLSDGKR HDVRAPDYDD WSTSGESERA GLNGDILVWN 250 260 270 280 290 300 PILEDAFELS SMGIRVDADA LKRQLAVTGD EDRLKLEWHQ ALLRGEMPQT IGGGIGQSRL 310 320 330 TMLLLQLSHI GQVQCGVWPQ QVRASVDSLL

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP62773.1.
RefSeq	YP_001178824.1. NC_009436.1.
3D structure databases
ProteinModelPortal	A4WGE3.
SMR	A4WGE3. Positions 4-330.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WGE3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5110691.
GenomeReviews	Gene locus Ent638_4120 in contig CP000653_GR.
KEGG	ent:Ent638_4120.
NMPDR	fig|399742.4.peg.3917.
PATRIC	20418035. VBIEntSp101211_4286.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	A4WGE3.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	ESP42895:ENT638_4120-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_ENT38

Accession

Primary (citable) accession number: A4WGE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program