A4WFX5 (FADA_ENT38) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-ketoacyl-CoA thiolase
EC=2.3.1.16

Alternative name(s):
Acetyl-CoA acyltransferase
Beta-ketothiolase
Fatty acid oxidation complex subunit beta

Gene names

Name:	fadA
Ordered Locus Names:	Ent638_3950

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed By similarity. HAMAP MF_01620
Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA. HAMAP MF_01620
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01620
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01620.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	3-ketoacyl-CoA thiolase HAMAP MF_01620		PRO_0000323544
Sites
	Active site	91	1	Acyl-thioester intermediate By similarity
	Active site	343	1	Proton acceptor By similarity
	Active site	373	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WFX5 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: BA35045BFA3417AB	FASTA	387	41,187
	10 20 30 40 50 60 MEKVVIVDAI RTPMGRSKGG AFRHVRAEDL SAHLMRSLLA RNPALEASAI DDIYWGCVQQ 70 80 90 100 110 120 TLEQGFNIAR NASLLAEIPH SVPAVTVNRL CGSSMQALHD AARMIMTGDA QVCMVGGVEH 130 140 150 160 170 180 MGHVPMSHGV DFHPGMSRNV AKAAGMMGLT AEMLSRMHGI SREMQDQFAA RSHARAWAAT 190 200 210 220 230 240 QSGAFKNEIL PTGGHDVDGV LKQYYYDEVI RPETTVEALS ALRPAFDPVT GTVTAGTSSA 250 260 270 280 290 300 LSDGAAAMLV MSESRARELG LTPRARIRSM AVVGCDPSIM GYGPVPASKL ALKKAGLTAS 310 320 330 340 350 360 EIDVFEMNEA FAAQILPCIK DLGLMEQIDE KINLNGGAIA LGHPLGCSGA RISTTLINLM 370 380 ERKDAQFGLA TMCIGLGQGI ATVFERV

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP62605.1.
RefSeq	YP_001178656.1. NC_009436.1.
3D structure databases
ProteinModelPortal	A4WFX5.
SMR	A4WFX5. Positions 4-387.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WFX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5114670.
GenomeReviews	Gene locus Ent638_3950 in contig CP000653_GR.
KEGG	ent:Ent638_3950.
NMPDR	fig|399742.4.peg.3751.
PATRIC	20417661. VBIEntSp101211_4114.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0183.
HOGENOM	HBG370930.
OMA	AIDDIYW.
PhylomeDB	A4WFX5.
ProtClustDB	PRK08947.
Enzyme and pathway databases
BioCyc	ESP42895:ENT638_3950-MONOMER.
Family and domain databases
HAMAP	MF_01620. FadA. [Tree]
InterPro	IPR012805. FadA. IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00632.
PANTHER	PTHR18919:SF35. PTHR18919:SF35. 1 hit. PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit. TIGR02445. FadA. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADA_ENT38

Accession

Primary (citable) accession number: A4WFX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program