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A4WFL5 (GLGB_ENT38) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Ent638_3840
OrganismEnterobacter sp. (strain 638) [Complete proteome] [HAMAP]
Taxonomic identifier399742 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

Protein attributes

Sequence length728 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7287281,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000061991

Sites

Active site4051Nucleophile By similarity
Active site4581Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A4WFL5 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 13A2B2CE383AF71F

FASTA72883,912
        10         20         30         40         50         60 
MSDRIDRDVI NALIAGHFSD PFSVLGMHPT EAGVEVRALL PDATEVWVIE PKTGRKVGKL 

        70         80         90        100        110        120 
ECLDSRGFFS GVIPRRKNIF RYQLAVLWHG QENLIDDPYS FGPLIQEMDA WLLSEGTHLR 

       130        140        150        160        170        180 
PYETLGAHAD TMDGITGTRF SVWAPNAQRV SVVGQFNYWD GRRHPMRLRR ESGIWELFIP 

       190        200        210        220        230        240 
GAHNGQLYKF EMIDAHGKLR VKADPYAFEA QMRPATASLI CGLPEKVVQS EERKQANNFD 

       250        260        270        280        290        300 
APISIYEVHL GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHIELLPIN EHPFDGSWGY 

       310        320        330        340        350        360 
QPTGLYAPTR RFGTRDDFRY FINAAHAAGL SVILDWVPGH FPSDDFGLSE FDGTDLYEHS 

       370        380        390        400        410        420 
DPREGYHQDW NTLIYNYGRR EVANYLVGNA LYWIERFGID ALRVDAVASM IYRDYSRKEG 

       430        440        450        460        470        480 
EWIPNEYGGR ENLEAIEFLR NTNRILGEQV PGAVTMAEES TDFPGVSRPP SMGGLGFWYK 

       490        500        510        520        530        540 
WNLGWMHDTL DYFKLDPVFR KYHHDKLTFG MLYNNTENFV LPLSHDEVVH GKKSILDRMP 

       550        560        570        580        590        600 
GDAWQKFANL RAYYGWMFAF PGKKLLFMGN EFAQGREWNH DSSLDWHLLE GGDNWHHGVQ 

       610        620        630        640        650        660 
RLVRDLNLTY RHHKALHEMD FDSYGFEWLV VDDHERSVFV FVRRDSAGNE IIVASNFTPV 

       670        680        690        700        710        720 
PRPHYRFGIN QPGKWREILN TDSSHYHGSN AGNAGAVQSD EHESHGRPHS LSLTLPPLST 


IWLVREGE 

« Hide

References

[1]"Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 638.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000653 Genomic DNA. Translation: ABP62495.1.
RefSeqYP_001178546.1. NC_009436.1.

3D structure databases

ProteinModelPortalA4WFL5.
SMRA4WFL5. Positions 117-728.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399742.Ent638_3840.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEA4WFL5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP62495; ABP62495; Ent638_3840.
GeneID5110884.
KEGGent:Ent638_3840.
PATRIC20417427. VBIEntSp101211_4002.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycESP399742:GJ0E-3928-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_ENT38
AccessionPrimary (citable) accession number: A4WFL5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries