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A4WFL5

- GLGB_ENT38

UniProt

A4WFL5 - GLGB_ENT38

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei405 – 4051NucleophileUniRule annotation
    Active sitei458 – 4581Proton donorUniRule annotation

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciESP399742:GJ0E-3928-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
    Alpha-(1->4)-glucan branching enzymeUniRule annotation
    Glycogen branching enzymeUniRule annotation
    Short name:
    BEUniRule annotation
    Gene namesi
    Name:glgBUniRule annotation
    Ordered Locus Names:Ent638_3840
    OrganismiEnterobacter sp. (strain 638)
    Taxonomic identifieri399742 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
    ProteomesiUP000000230: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7287281,4-alpha-glucan branching enzyme GlgBPRO_1000061991Add
    BLAST

    Proteomic databases

    PRIDEiA4WFL5.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi399742.Ent638_3840.

    Structurei

    3D structure databases

    ProteinModelPortaliA4WFL5.
    SMRiA4WFL5. Positions 117-728.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiGELSHAW.
    OrthoDBiEOG6JX7GT.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4WFL5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDRIDRDVI NALIAGHFSD PFSVLGMHPT EAGVEVRALL PDATEVWVIE    50
    PKTGRKVGKL ECLDSRGFFS GVIPRRKNIF RYQLAVLWHG QENLIDDPYS 100
    FGPLIQEMDA WLLSEGTHLR PYETLGAHAD TMDGITGTRF SVWAPNAQRV 150
    SVVGQFNYWD GRRHPMRLRR ESGIWELFIP GAHNGQLYKF EMIDAHGKLR 200
    VKADPYAFEA QMRPATASLI CGLPEKVVQS EERKQANNFD APISIYEVHL 250
    GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHIELLPIN EHPFDGSWGY 300
    QPTGLYAPTR RFGTRDDFRY FINAAHAAGL SVILDWVPGH FPSDDFGLSE 350
    FDGTDLYEHS DPREGYHQDW NTLIYNYGRR EVANYLVGNA LYWIERFGID 400
    ALRVDAVASM IYRDYSRKEG EWIPNEYGGR ENLEAIEFLR NTNRILGEQV 450
    PGAVTMAEES TDFPGVSRPP SMGGLGFWYK WNLGWMHDTL DYFKLDPVFR 500
    KYHHDKLTFG MLYNNTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL 550
    RAYYGWMFAF PGKKLLFMGN EFAQGREWNH DSSLDWHLLE GGDNWHHGVQ 600
    RLVRDLNLTY RHHKALHEMD FDSYGFEWLV VDDHERSVFV FVRRDSAGNE 650
    IIVASNFTPV PRPHYRFGIN QPGKWREILN TDSSHYHGSN AGNAGAVQSD 700
    EHESHGRPHS LSLTLPPLST IWLVREGE 728
    Length:728
    Mass (Da):83,912
    Last modified:May 29, 2007 - v1
    Checksum:i13A2B2CE383AF71F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP62495.1.
    RefSeqiWP_015960800.1. NC_009436.1.
    YP_001178546.1. NC_009436.1.

    Genome annotation databases

    EnsemblBacteriaiABP62495; ABP62495; Ent638_3840.
    GeneIDi5110884.
    KEGGient:Ent638_3840.
    PATRICi20417427. VBIEntSp101211_4002.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP62495.1 .
    RefSeqi WP_015960800.1. NC_009436.1.
    YP_001178546.1. NC_009436.1.

    3D structure databases

    ProteinModelPortali A4WFL5.
    SMRi A4WFL5. Positions 117-728.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399742.Ent638_3840.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PRIDEi A4WFL5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP62495 ; ABP62495 ; Ent638_3840 .
    GeneIDi 5110884.
    KEGGi ent:Ent638_3840.
    PATRICi 20417427. VBIEntSp101211_4002.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi GELSHAW.
    OrthoDBi EOG6JX7GT.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci ESP399742:GJ0E-3928-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
      Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
      PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 638.

    Entry informationi

    Entry nameiGLGB_ENT38
    AccessioniPrimary (citable) accession number: A4WFL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3