Reviewed, UniProtKB/Swiss-Prot A4WE75 (SPEB_ENT38)

Names and origin

Protein names

Recommended name:
    Agmatinase
    EC=3.5.3.11
Alternative name(s):
    Agmatine ureohydrolase
      Short name=AUH

Gene names

Name:	speB
Ordered Locus Names:	Ent638_3343

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of putrescine from agmatine By similarity. HAMAP MF_01418
Catalytic activity	Agmatine + H2O = putrescine + urea. HAMAP MF_01418
Cofactor	Manganese By similarity. HAMAP MF_01418
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1. HAMAP MF_01418
Sequence similarities	Belongs to the arginase family. Agmatinase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	putrescine biosynthetic process Inferred from electronic annotation. Source: HAMAP spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	agmatinase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Agmatinase HAMAP MF_01418		PRO_1000068496
Sites
	Metal binding	126	1	Manganese By similarity
	Metal binding	149	1	Manganese By similarity
	Metal binding	151	1	Manganese By similarity
	Metal binding	153	1	Manganese By similarity
	Metal binding	230	1	Manganese By similarity
	Metal binding	232	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WE75-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 5B8DFD35A898C936	FASTA	306	33,481
	10 20 30 40 50 60 MSTLGHQYDN SLVSNAFGFL RLPMNFQPYD SDADWVITGV PFDMATSGRA GGRHGPAAIR 70 80 90 100 110 120 QVSTNLAWEH NRFPWNFDMR ERLNVVDCGD LVYAFGDARE MSEKLQAHAE KLLAAGKRML 130 140 150 160 170 180 SFGGDHFVTL PLLRAHAKHF GKMALVHFDA HTDTYANGCE FDHGTMFYTA PNEGLIDPNH 190 200 210 220 230 240 SVQIGIRTEF DKDNGFTVLD AGQVNDRGVD DIIAQVKQIV GDMPVYLTFD IDCLDPAFAP 250 260 270 280 290 300 GTGTPVIGGL TSDRAIKLVR GLKDLNIVGM DVVEVAPAYD QSEITALAAA TLALEMLYIQ AAKKGE

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP62005.1.
RefSeq	YP_001178056.1.
3D structure databases
SMR	A4WE75. Positions 17-301.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WE75.
Genome annotation databases
GeneID	5111857.
GenomeReviews	Gene locus Ent638_3343 in contig CP000653_GR.
KEGG	ent:Ent638_3343.
NMPDR	fig|399742.4.peg.3184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0010.
HOGENOM	HBG391953.
OMA	DCAQITA.
PhylomeDB	A4WE75.
Family and domain databases
HAMAP	MF_01418. SpeB. [Tree]
InterPro	IPR005925. Agmatinase. IPR006035. Ureohydrolase. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01230. agmatinase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEB_ENT38

Accession

Primary (citable) accession number: A4WE75

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)