A4WE72 (E4PD_ENT38) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-erythrose-4-phosphate dehydrogenase
Short name=E4PDH
EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	Ent638_3340

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity. HAMAP MF_01640
Catalytic activity	D-erythrose 4-phosphate + NAD+ + H2O = 4-phosphoerythronate + NADH. HAMAP MF_01640
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5. HAMAP MF_01640
Subunit structure	Homotetramer By similarity. HAMAP MF_01640
Subcellular location	Cytoplasm By similarity HAMAP MF_01640.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: InterPro pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	D-erythrose-4-phosphate dehydrogenase HAMAP MF_01640		PRO_1000069894
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	154 – 156	3	Substrate binding Potential
	Region	213 – 214	2	Substrate binding Potential
Sites
	Active site	155	1	Nucleophile By similarity
	Binding site	200	1	Substrate Potential
	Binding site	236	1	Substrate Potential
	Binding site	318	1	NAD By similarity
	Site	182	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WE72 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: F1E4C31BEFFF7A1B	FASTA	339	37,248
	10 20 30 40 50 60 MTVRVAINGF GRIGRNVIRA LYESGRRAEI TVVAINELAD AVGMAHLLKY DTSHGRFAWD 70 80 90 100 110 120 VRQEREQLFV GDDAIRVLHE QSIDALPWRE LGVDVVLDCT GVYGNREHGE AHLAAGAKKV 130 140 150 160 170 180 LFSHPGGHDL DATVVYGVNH HELQAEHRIV SNASCTTNCI IPVIKLLDDA YGIESGTVTT 190 200 210 220 230 240 IHSAMHDQQV IDAYHPDLRR TRAASQSIIP VDTKLAAGIT RIFPQFNDRF EAIAVRVPTI 250 260 270 280 290 300 NVTAIDLSVT VKKPVKASEV NLLLQKAAQG SFHGIVDYTE LPLVSVDFNH DPHSAIVDGT 310 320 330 QTRVSGAHLI KTLVWCDNEW GFANRMLDTT LAMAAQGFR

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP62002.1.
RefSeq	YP_001178053.1. NC_009436.1.
3D structure databases
ProteinModelPortal	A4WE72.
ModBase	Search...
Protein-protein interaction databases
STRING	A4WE72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5111854.
GenomeReviews	Gene locus Ent638_3340 in contig CP000653_GR.
KEGG	ent:Ent638_3340.
NMPDR	fig|399742.4.peg.3181.
PATRIC	20416403. VBIEntSp101211_3500.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0057.
HOGENOM	HBG571736.
OMA	TTHGRFQ.
PhylomeDB	A4WE72.
ProtClustDB	PRK13535.
Enzyme and pathway databases
BioCyc	ESP42895:ENT638_3340-MONOMER.
Family and domain databases
HAMAP	MF_01640. E4P_dehydrog. [Tree]
InterPro	IPR006422. E4P_DH_bac. IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K03472.
PANTHER	PTHR10836. GAP_DH. 1 hit. PTHR10836:SF24. PTHR10836:SF24. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit. TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_ENT38

Accession

Primary (citable) accession number: A4WE72

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program