Reviewed, UniProtKB/Swiss-Prot A4WDW1 (CYSJ_ENT38)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] flavoprotein alpha-component
      Short name=SIR-FP
    EC=1.8.1.2

Gene names

Name:	cysJ
Ordered Locus Names:	Ent638_3227

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavo-protein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 FAD per subunit By similarity. Binds 1 FMN per subunit By similarity.
Subunit structure	Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Electron transport Transport
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Sulfite reductase [NADPH] flavoprotein alpha-component		PRO_1000087635
Regions
	Domain	64 – 202	139	Flavodoxin-like
	Domain	236 – 450	215	FAD-binding FR-type
	Nucleotide binding	70 – 74	5	FMN By similarity
	Nucleotide binding	150 – 181	32	FMN By similarity
	Nucleotide binding	238 – 290	53	FAD By similarity
	Nucleotide binding	474 – 601	128	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WDW1-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: D4C25C7138BF8496	FASTA	601	66,366
	10 20 30 40 50 60 MTTQAPPSNL LPLNPEQLAR LQAATTDFSP TQLAWVSGYF WGMLNQQPGA VVNAPATAVE 70 80 90 100 110 120 IPAITLISAS QTGNARRVAE ALRDDLLAAK LNVNLVNAGD YKFKQIASEK LVVVVASTQG 130 140 150 160 170 180 EGEPAEEAVA LHKFLFSKKA PKLDGTAFAV FGLGDTSYEF FCQSGKDFDS KLAELGAERL 190 200 210 220 230 240 LDRVDADVEY QAAAAEWRAR IVDVLKARVP KDTPAQAANS ASGAVNEVST SPYTKEEPLV 250 260 270 280 290 300 ASLSVNQKIT GRDSEKDVRH IEIDLGDSGL RYQPGDALGV WYQNDPALVK ELVELLWLKG 310 320 330 340 350 360 TEQVNVEGKT LPLSEALQWH FELTVNTANI VENYATLTRS ETLLPLVGDK AKLQHYAATT 370 380 390 400 410 420 PIVDMVRFSP AQLDAEALIG LLRPLTPRLY SIASSQAEVE SEVHITVGAV RFDIEGRARA 430 440 450 460 470 480 GGASSFLADR VEEEGEVRVF IEHNDNFRLP ANPETPVIMI GPGTGIAPFR AFMQQRAAEE 490 500 510 520 530 540 APGKNWLFFG NPHFTEDFLY QVEWQRYVKE GVLSRIDLAW SRDQKQKIYV QDKLREQGAE 550 560 570 580 590 600 LWAWINNGAH LYVCGDANRM AKDVEQALLE VIAEFGGMDI ETADEFLSEL RVERRYQRDV Y

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP61891.1.
RefSeq	YP_001177942.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5112941.
GenomeReviews	Gene locus Ent638_3227 in contig CP000653_GR.
KEGG	ent:Ent638_3227.
NMPDR	fig|399742.4.peg.3073.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01541. [Tree]
InterPro	IPR010199. CysJ. IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
TIGRFAMs	TIGR01931. cysJ. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSJ_ENT38

Accession

Primary (citable) accession number: A4WDW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)