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Protein

Ribonuclease 3

Gene

rnc

Organism
Enterobacter sp. (strain 638)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411MagnesiumUniRule annotation
Active sitei45 – 451UniRule annotation
Metal bindingi114 – 1141MagnesiumUniRule annotation
Active sitei117 – 1171UniRule annotation
Metal bindingi117 – 1171MagnesiumUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciESP399742:GJ0E-3118-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:Ent638_3054
OrganismiEnterobacter sp. (strain 638)
Taxonomic identifieri399742 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
ProteomesiUP000000230 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Ribonuclease 3PRO_1000075752Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi399742.Ent638_3054.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 128123RNase IIIUniRule annotationAdd
BLAST
Domaini155 – 22571DRBMUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
Contains 1 RNase III domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246809.
KOiK03685.
OMAiMTGGRDK.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4WDD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPIVINRLQ RKLGYTFQHH ELLQQALTHR SASSKHNERL EFLGDSILSY
60 70 80 90 100
VIANALYHRF PRVDEGDMSR MRATLVRGNT LAEIAREFEL GECLRLGPGE
110 120 130 140 150
LKSGGFRRES ILADTVEALI GGVFLDSDIQ TVEQLILNWY QTRLDEISPG
160 170 180 190 200
DKQKDPKTRL QEYLQGRHLP LPSYLVVQVR GEAHDQEFTI HCQVSGLSEP
210 220
VVGTGSSRRK AEQAAAEQAL KMLELE
Length:226
Mass (Da):25,562
Last modified:May 28, 2007 - v1
Checksum:i3B0D27813E25B340
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP61718.1.
RefSeqiWP_015960048.1. NC_009436.1.
YP_001177769.1. NC_009436.1.

Genome annotation databases

EnsemblBacteriaiABP61718; ABP61718; Ent638_3054.
KEGGient:Ent638_3054.
PATRICi20415787. VBIEntSp101211_3205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP61718.1.
RefSeqiWP_015960048.1. NC_009436.1.
YP_001177769.1. NC_009436.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399742.Ent638_3054.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP61718; ABP61718; Ent638_3054.
KEGGient:Ent638_3054.
PATRICi20415787. VBIEntSp101211_3205.

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246809.
KOiK03685.
OMAiMTGGRDK.
OrthoDBiEOG6T1WVS.

Enzyme and pathway databases

BioCyciESP399742:GJ0E-3118-MONOMER.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
    Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
    PLoS Genet. 6:E1000943-E1000943(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 638.

Entry informationi

Entry nameiRNC_ENT38
AccessioniPrimary (citable) accession number: A4WDD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 19, 2008
Last sequence update: May 28, 2007
Last modified: March 31, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.