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A4WCW6

- FADJ_ENT38

UniProt

A4WCW6 - FADJ_ENT38

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei118 – 1181Important for catalytic activityUniRule annotation
    Sitei140 – 1401Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciESP399742:GJ0E-2938-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:Ent638_2881
    OrganismiEnterobacter sp. (strain 638)
    Taxonomic identifieri399742 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
    ProteomesiUP000000230: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 715715Fatty acid oxidation complex subunit alphaPRO_1000069486Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi399742.Ent638_2881.

    Structurei

    3D structure databases

    ProteinModelPortaliA4WCW6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 190190Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni306 – 7154103-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OMAiPFRYMDT.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4WCW6-1 [UniParc]FASTAAdd to Basket

    « Hide

    METTSAFTLN VRLDNIAVVT IDVPGEKMNT LKAEFGVQVR SILKQVRDNK    50
    NIRGLIFISA KSDNFIAGAD INMIARAKSA QEAEELARQG QQIMAEIHGL 100
    SIPVIAAIHG ACLGGGLELA LACHSRVCTD DAKTVLGLPE VQLGLLPGSG 150
    GTQRLPRLVG VSTALEMILT GKQLRPRQAL KVGLVDEVVP HSILLTAAVE 200
    LAQKERQASR HLPVRERILA GPLGRALLFN RVGKKTEQKT KGNYPATKRI 250
    LDVIETGLSQ GSSSGYAAEA KAFGELAMTP QSQALRSIFF ASTEVKKDPG 300
    SEVAPGPLNA IGVLGGGLMG GGISFVTASK GKLPVRIKDI NAKGINHALQ 350
    YSWQLLDQKV KRRHIKASER DRALALISGT IDFSGFKHRD VVIEAVFEDL 400
    QLKQQMVADV EQYCAPHTIF ASNTSSLPIG DIAANAARPE QVIGLHFFSP 450
    VEKMPLVEVI PHESTSAQTV ATVVKLAKRQ GKTPIVVADK AGFYVNRILA 500
    PYINEAMRLL TEGEKVENVD DALVKFGFPV GPIQLLDEVG IDTGTKIIPV 550
    LEAAYGERFS PPANVVSAIL NDDRKGRKNG RGFYLYAAKG RKSKKQVDPS 600
    VYGLINASGQ GKLTAQQCAE RCVMMMLNEA ARCFDEKVIK NARDGDIGAV 650
    FGIGFPPFLG GPFRYMDSLG AGEVVATLQR LASLYGSRFT PCETLKQMAE 700
    NDLRFWPAQE TDPIN 715
    Length:715
    Mass (Da):77,129
    Last modified:May 29, 2007 - v1
    Checksum:i0AE6C76B7C74B668
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP61546.1.
    RefSeqiWP_015959879.1. NC_009436.1.
    YP_001177597.1. NC_009436.1.

    Genome annotation databases

    EnsemblBacteriaiABP61546; ABP61546; Ent638_2881.
    GeneIDi5111426.
    KEGGient:Ent638_2881.
    PATRICi20415427. VBIEntSp101211_3032.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP61546.1 .
    RefSeqi WP_015959879.1. NC_009436.1.
    YP_001177597.1. NC_009436.1.

    3D structure databases

    ProteinModelPortali A4WCW6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399742.Ent638_2881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP61546 ; ABP61546 ; Ent638_2881 .
    GeneIDi 5111426.
    KEGGi ent:Ent638_2881.
    PATRICi 20415427. VBIEntSp101211_3032.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OMAi PFRYMDT.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci ESP399742:GJ0E-2938-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
      Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
      PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 638.

    Entry informationi

    Entry nameiFADJ_ENT38
    AccessioniPrimary (citable) accession number: A4WCW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3